检测摩洛哥鼻咽癌患者的人乳头瘤病毒。
Human papillomavirus detection in moroccan patients with nasopharyngeal carcinoma.
机构信息
Laboratory of Oncovirology, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20 360 Casablanca, Morocco.
出版信息
Infect Agent Cancer. 2011 Feb 25;6(1):3. doi: 10.1186/1750-9378-6-3.
BACKGROUND
Nasopharyngeal carcinoma (NPC) is a malignant tumor which arises in surface epithelium of the posterior wall of the nasopharynx. There's is evidence that Epstein Barr virus (EBV) is associated to NPC development. However, many epidemiologic studies point to a connection between viral infections by the human papillomavirus (HPV) and NPC.
METHOD
Seventy Moroccan patients with NPC were screened for EBV and HPV. EBV detection was performed by PCR amplification of BZLF1 gene, encoding the ZEBRA (Z Epstein-Barr Virus Replication Activator) protein, and HPV infection was screened by PCR amplification with subsequent typing by hybridization with specific oligonucleotides for HPV types 16, 18, 31, 33, 35, 45 and 59.
RESULTS
The age distribution of our patients revealed a bimodal pattern. Sixty two cases (88.9%) were classified as type 3 (undifferentiated carcinoma), 6 (8.6%) as type 2 (non keratinizing NPC) and only 2 (2.9%) cases were classified as type 1 (keratinizing NPC). EBV was detected in all NPC tumors, whereas HPV DNA was revealed in 34% of cases (24/70). Molecular analysis showed that 20.8% (5/24) were infected with HPV31, and the remaining were infected with other oncogenic types (i.e., HPV59, 16, 18, 33, 35 and 45). In addition, statistical analysis showed that there's no association between sex or age and HPV infection (P > 0.1).
CONCLUSION
Our data indicated that EBV is commonly associated with NPC in Moroccan patients and show for the first time that NPC tumours from Moroccan patients harbour high risk HPV genotypes.
背景
鼻咽癌（NPC）是一种恶性肿瘤，起源于鼻咽后壁的表面上皮。有证据表明， Epstein Barr 病毒（EBV）与 NPC 的发展有关。然而，许多流行病学研究表明，人类乳头瘤病毒（HPV）的病毒感染与 NPC 之间存在联系。
方法
对 70 名 NPC 摩洛哥患者进行 EBV 和 HPV 筛查。通过 BZLF1 基因的 PCR 扩增检测 EBV，该基因编码 ZEBRA（Z Epstein-Barr 病毒复制激活剂）蛋白，通过 PCR 扩增并与特定的 HPV 类型 16、18、31、33、35、45 和 59 的寡核苷酸杂交来筛选 HPV 感染。
结果
我们患者的年龄分布呈双峰模式。62 例（88.9%）被归类为 3 型（未分化癌），6 例（8.6%）为 2 型（非角化 NPC），只有 2 例（2.9%）被归类为 1 型（角化 NPC）。所有 NPC 肿瘤中均检测到 EBV，而 HPV DNA 则在 34%的病例中（24/70）被发现。分子分析显示，20.8%（5/24）感染 HPV31，其余感染其他致癌类型（即 HPV59、16、18、33、35 和 45）。此外，统计分析表明，HPV 感染与性别或年龄无关（P>0.1）。
结论
我们的数据表明，EBV 通常与摩洛哥患者的 NPC 相关，并首次表明来自摩洛哥患者的 NPC 肿瘤携带高危 HPV 基因型。
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