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本文引用的文献

1
Line probe and automated real-time PCR TB assays warrant adherence to strict quality assurance measures.线性探针和自动化实时聚合酶链反应结核检测需要严格遵守质量保证措施。
Int J Tuberc Lung Dis. 2012 May;16(5):705-6. doi: 10.5588/ijtld.12.0078.
2
Comparison of two nucleic acid amplification assays, the Xpert MTB/RIF assay and the amplified Mycobacterium Tuberculosis Direct assay, for detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens.比较两种核酸扩增检测方法，Xpert MTB/RIF 检测法和扩增分枝杆菌直接检测法，用于检测呼吸道和非呼吸道标本中的结核分枝杆菌。
J Clin Microbiol. 2011 Oct;49(10):3659-62. doi: 10.1128/JCM.00211-11. Epub 2011 Aug 24.
3
Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study.基层应用 Xpert MTB/RIF 检测诊断结核分枝杆菌感染和耐药的可行性、诊断准确性和效果：一项多中心实施研究。
Lancet. 2011 Apr 30;377(9776):1495-505. doi: 10.1016/S0140-6736(11)60438-8. Epub 2011 Apr 18.
4
Evaluation of the Xpert MTB/RIF assay for the diagnosis of pulmonary tuberculosis in a high HIV prevalence setting.评估 Xpert MTB/RIF assay 在高 HIV 流行环境下用于诊断肺结核的效果。
Am J Respir Crit Care Med. 2011 Jul 1;184(1):132-40. doi: 10.1164/rccm.201101-0056OC. Epub 2011 Apr 14.
5
Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens.比较 Xpert MTB/RIF 检测与 IS6110-TaqMan 实时 PCR 检测在呼吸道和非呼吸道标本中直接检测结核分枝杆菌的效果。
J Clin Microbiol. 2011 May;49(5):1772-6. doi: 10.1128/JCM.02157-10. Epub 2011 Mar 16.
6
Rapid molecular detection of tuberculosis.结核病的快速分子检测
N Engl J Med. 2011 Jan 13;364(2):183; author reply 184-5. doi: 10.1056/NEJMc1011919.
7
Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method.采用整合实时 PCR 方法快速检测涂阴临床标本中的结核分枝杆菌复合群和利福平耐药性。
J Clin Microbiol. 2011 Mar;49(3):1137-9. doi: 10.1128/JCM.01831-10. Epub 2010 Dec 29.
8
Evaluation of the Cobas TaqMan MTB test for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.评价 Cobas TaqMan MTB 试验直接检测呼吸道标本中的结核分枝杆菌复合群。
J Clin Microbiol. 2011 Mar;49(3):797-801. doi: 10.1128/JCM.01839-10. Epub 2010 Dec 22.
9
Rapid molecular detection of tuberculosis and rifampin resistance.快速分子检测结核分枝杆菌及利福平耐药性。
N Engl J Med. 2010 Sep 9;363(11):1005-15. doi: 10.1056/NEJMoa0907847. Epub 2010 Sep 1.
10
Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology.采用按需、近患者技术快速检测结核分枝杆菌和利福平耐药性。
J Clin Microbiol. 2010 Jan;48(1):229-37. doi: 10.1128/JCM.01463-09. Epub 2009 Oct 28.

将 Xpert MTB/RIF assay 整合到诊断工作流程中，用于快速检测低流行地区的结核分枝杆菌。

Integrating the Xpert MTB/RIF assay into a diagnostic workflow for rapid detection of Mycobacterium tuberculosis in a low-prevalence area.

机构信息

Institut für Medizinische Mikrobiologie, Universität Zürich, Zürich, Switzerland.

出版信息

J Clin Microbiol. 2013 Jul;51(7):2396-9. doi: 10.1128/JCM.00151-13. Epub 2013 Apr 24.

DOI:10.1128/JCM.00151-13
PMID:23616455
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3697699/
Abstract

The Xpert MTB/RIF assay is a rapid and fully automated real-time PCR assay. The performance of the Xpert MTB/RIF assay as a primary screening test for urgent clinical specimens was evaluated during a 2-year period. The results showed that replacing smear microscopy with the Xpert MTB/RIF assay facilitates laboratory handling and improves the sensitivity and specificity of Mycobacterium tuberculosis detection.

摘要

Xpert MTB/RIF assay 是一种快速且全自动的实时 PCR 检测方法。在为期两年的研究期间，评估了 Xpert MTB/RIF assay 作为紧急临床标本一线筛查检测的性能。结果表明，用 Xpert MTB/RIF assay 取代痰涂片显微镜检查，有利于实验室处理，并且提高了结核分枝杆菌检测的敏感性和特异性。