Instituto de Biotecnologia e Bioengenharia, Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Carbohydr Polym. 2013 Jun 5;95(1):134-42. doi: 10.1016/j.carbpol.2013.02.067. Epub 2013 Mar 6.
In this work three different hydrogels were developed to associate, as vehicles, with the synthetic bone substitute GR-HAP. One based on an alginate matrix (Alg); a second on a mixture of alginate and chitosan (Alg/Ch); and a third on alginate and hyaluronate (Alg/HA), using Ca(2+) ions as cross-linking agents. The hydrogels, as well as the respective injectable bone substitutes (IBSs), were fully characterized from the physical-chemical point of view. Weight change studies proved that all hydrogels were able to swell and degrade within 72 h at pH 7.4 and 4.0, being Alg/HA the hydrogel with the highest degradation rate (80%). Rheology studies demonstrated that all hydrogels are non-Newtonian viscoelastic fluids, and injectability tests showed that IBSs presented low maximum extrusion forces, as well as quite stable average forces. In conclusion, the studied hydrogels present the necessary features to be successfully used as vehicles of GR-HAP, particularly the hydrogel Alg/HA.
在这项工作中，开发了三种不同的水凝胶，以作为载体与合成骨替代物 GR-HAP 结合。一种基于海藻酸钠基质（Alg）；另一种是海藻酸钠和壳聚糖的混合物（Alg/Ch）；第三种是海藻酸钠和透明质酸钠（Alg/HA），使用 Ca(2+)离子作为交联剂。从物理化学的角度对水凝胶以及各自的可注射骨替代物（IBS）进行了全面表征。重量变化研究证明，所有水凝胶在 pH 7.4 和 4.0 下均能在 72 小时内溶胀和降解，Alg/HA 是降解速度最快的水凝胶（80%）。流变学研究表明，所有水凝胶均为非牛顿粘性弹性流体，注射性测试表明 IBS 具有较低的最大挤出力以及相当稳定的平均力。总之，研究的水凝胶具有成功用作 GR-HAP 载体的必要特性，特别是 Alg/HA 水凝胶。
