Zalewska Anna, Knaś Małgorzata, Gumiężny Grzegorz, Niczyporuk Marek, Waszkiel Danuta, Przystupa Adrian Wojciech, Zarzycki Wiesław
Department of Pedodontics, Medical University of Bialystok, 15-274 Bialystok, Poland.
Postepy Hig Med Dosw (Online). 2013 Apr 19;67:315-20. doi: 10.5604/17322693.1046007.
As exoglycosidases have been described as potential markers of salivary gland pathology, we decided to check the possibility of the use of these enzymes in the detection of salivary gland involvement in gestational diabetes.
For this purpose diabetic pregnant women were compared to pregnant and non-pregnant healthy women. The activities of total HEX as well as GLU in the saliva were determined in duplicate according to Marciniak et al. The activities of GAL, FUC, and MAN in the saliva were determined in duplicate according to Zwierz et al.
It was found that the specific activities of exoglycosidases in the saliva of diabetic pregnant women significantly increased in comparison to healthy pregnant and non-pregnant women.
Increased specific activity of exoglycosidases suggests that gestational diabetes provokes structural/functional alterations in salivary glands and changes in the salivary glycoconjugates metabolism.
由于外切糖苷酶已被描述为唾液腺病理学的潜在标志物，我们决定研究使用这些酶检测妊娠糖尿病患者唾液腺受累情况的可能性。
为此，将糖尿病孕妇与健康孕妇及非孕妇进行比较。根据Marciniak等人的方法，对唾液中总己糖胺酶（HEX）以及β-葡萄糖苷酶（GLU）的活性进行了双份测定。根据Zwierz等人的方法，对唾液中半乳糖苷酶（GAL）、岩藻糖苷酶（FUC）和甘露糖苷酶（MAN）的活性进行了双份测定。
发现与健康孕妇和非孕妇相比，糖尿病孕妇唾液中外切糖苷酶的比活性显著增加。
外切糖苷酶比活性的增加表明妊娠糖尿病会引发唾液腺的结构/功能改变以及唾液糖缀合物代谢的变化。
