Department of Urology, Zhongnan Hospital, Wuhan University, 169 Donghu Road, Wuhan, China,
Ir J Med Sci. 2013 Dec;182(4):621-7. doi: 10.1007/s11845-013-0941-y. Epub 2013 Apr 26.
The aim of this study was to determine the expression and function of miR-509-5p in renal cell carcinoma (RCC).
In this research, we have conducted quantitative real-time polymerase chain reaction (qRT-PCR) assay to determine the expression level of miR-509-5p in tissues and plasma from renal cell carcinoma patients. We preformed in vitro migration scratch assay, flow cytometry analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the exact function of miR-509-5p.
We evaluated the expression level of miR-509-5p in RCC tissues and paired adjacent normal tissues from 42 patients and found that miR-509-5p expression in 42 RCC specimens was significantly down-regulated compared to that in adjacent normal tissue. Furthermore, the level of miR-509-5p in RCC patients' plasma was significantly lower than that in control plasma. In addition, the overexpression of miR-509-5p suppressed the proliferation of RCC cell (786-0), induced cell apoptosis and inhibited cell migration in vitro.
In this study, we have shown that miR-509-5p played an important role in RCC by inhibiting cell proliferation and migration and by promoting cell apoptosis. In addition, miR-509-5p expression was significantly lower in RCC patient plasma compared to that in normal individuals.
本研究旨在确定 miR-509-5p 在肾细胞癌（RCC）中的表达和功能。
在这项研究中，我们通过定量实时聚合酶链反应（qRT-PCR）测定了肾细胞癌患者组织和血浆中 miR-509-5p 的表达水平。我们进行了体外迁移划痕实验、流式细胞术分析和 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定，以确定 miR-509-5p 的确切功能。
我们评估了 42 例患者的 RCC 组织和配对的相邻正常组织中 miR-509-5p 的表达水平，发现 42 例 RCC 标本中 miR-509-5p 的表达明显低于相邻正常组织。此外，RCC 患者血浆中的 miR-509-5p 水平明显低于对照组血浆。此外，miR-509-5p 的过表达抑制了 RCC 细胞（786-0）的增殖，诱导细胞凋亡，并抑制了细胞的体外迁移。
在这项研究中，我们表明 miR-509-5p 通过抑制细胞增殖和迁移以及促进细胞凋亡在 RCC 中发挥重要作用。此外，与正常个体相比，RCC 患者血浆中的 miR-509-5p 表达明显降低。
