Laakso S, Scheinin M, Anttila M
Research Centre, Farmos Group Ltd., Turku, Finland.
J Chromatogr. 1990 Apr 6;526(2):475-86. doi: 10.1016/s0378-4347(00)82529-3.
A high-performance liquid chromatographic method for the determination of plasma concentrations of erythromycin base and 2'-acetylerythromycin, an ester prodrug of erythromycin, is described. tert.-Butyl methyl ether extracts of 1-ml plasma samples (pH 10) were chromatographed on a C18 reversed-phase column. A three-electrode coulometric detector (oxidation potentials +0.65 and +0.85 V) was used for quantitation. Oleandomycin was used as an internal standard. The method has good precision and accuracy, is linear in the range 0.25-7.5 mg/l and has proved to be suitable for pharmacokinetic studies in humans. Correlation with a microbiological assay was satisfactory (r greater than or equal to 0.95), but the chromatographic method gave ca. 30% higher values.
本文描述了一种高效液相色谱法，用于测定血浆中红霉素碱和红霉素酯前体药物2'-乙酰红霉素的浓度。取1 ml血浆样品（pH 10）用叔丁基甲醚萃取，然后在C18反相柱上进行色谱分析。采用三电极库仑检测器（氧化电位为+0.65和+0.85 V）进行定量分析。竹桃霉素用作内标。该方法具有良好的精密度和准确度，在0.25 - 7.5 mg/l范围内呈线性，已证明适用于人体药代动力学研究。与微生物测定法的相关性良好（r≥0.95），但色谱法测得的值约高30%。
