Croteau D, Vallée F, Bergeron M G, LeBel M
Université Laval, Centre Hospitalier, de l'Université Laval, Quebec, Canada.
J Chromatogr. 1987 Aug 7;419:205-12. doi: 10.1016/0378-4347(87)80278-5.
A high-performance liquid chromatographic analysis of erythromycin and its esters in plasma, urine and saliva is presented. A diethyl ether extract of sample was chromatographed on a reversed-phase column and components of the column effluent were monitored by electrochemical detection at +0.9 V (vs. Ag/AgCl). The method sensitivity limit was 10 ng with inter-day coefficients of variation from 3.2 to 10.3%. In order to assess precisely the relative concentrations of erythromycin esters (ethylsuccinate or estolate) and their active by-product erythromycin base, it is necessary to adopt measures preventing their continuous hydrolysis in biological fluids and during sample preparation.
本文介绍了血浆、尿液和唾液中红霉素及其酯类的高效液相色谱分析方法。样品的乙醚提取物在反相柱上进行色谱分离，柱流出物的成分通过在+0.9 V（相对于Ag/AgCl）下的电化学检测进行监测。该方法的灵敏度极限为10 ng，日间变异系数为3.2%至10.3%。为了精确评估红霉素酯（乙基琥珀酸酯或依托红霉素）及其活性副产物红霉素碱的相对浓度，有必要采取措施防止它们在生物流体和样品制备过程中持续水解。
