Chen M L, Chiou W L
J Chromatogr. 1983 Nov 11;278(1):91-100. doi: 10.1016/s0378-4347(00)84759-3.
A simple and sensitive high-performance liquid chromatographic assay was developed for the quantitative determination of major erythromycin components and their potential metabolites or degradation products in plasma and urine. An ether extract of alkalized plasma sample was chromatographed on a reversed-phase column and the components in the column effluent were monitored by an electrochemical detector. The recovery of the drug from extraction was virtually 100%. The detection limits for erythromycin A in plasma were 5-10 ng/ml and 30 ng/ml using 1 and 0.2 ml of sample, respectively. For urine samples, a simple one-step deproteinization with two volumes of acetonitrile was satisfactory for analysis. The method has been evaluated in plasma and urine from dogs receiving oral or intravenous erythromycin A. The standard curves for potential metabolites or degradation products were not constructed due to the lack of sufficient samples.
开发了一种简单灵敏的高效液相色谱法，用于定量测定血浆和尿液中主要红霉素成分及其潜在代谢物或降解产物。碱化血浆样品的乙醚提取物在反相柱上进行色谱分离，柱流出物中的成分通过电化学检测器进行监测。药物提取回收率几乎为100%。使用1ml和0.2ml样品时，血浆中红霉素A的检测限分别为5-10ng/ml和30ng/ml。对于尿液样品，用两倍体积的乙腈进行简单的一步脱蛋白处理即可满足分析要求。该方法已在口服或静脉注射红霉素A的犬的血浆和尿液中进行了评估。由于缺乏足够的样品，未构建潜在代谢物或降解产物的标准曲线。
