Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
Mater Sci Eng C Mater Biol Appl. 2013 Jul 1;33(5):2926-36. doi: 10.1016/j.msec.2013.03.020. Epub 2013 Mar 21.
The aim of this study was to compare physico-chemical and biological properties of hydroxyapatite (HA) and hardystonite (HS) based composite scaffolds. Hardystonite (Ca2ZnSi2O7) powders were synthesized by a sol-gel method while polycaprolactone-hardystonite (PCL-HS) and polycaprolactone-hydroxyapatite (PCL-HA) were fabricated in nanofibrous form by electrospinning. The physico-chemical and biological properties such as tensile strength, cell proliferation, cell infiltration and alkaline phosphatase activity were determined on both kinds of scaffolds. We found that PCL-HS scaffolds had better mechanical strength compared to PCL-HA scaffolds. Addition of HA and HS particles to PCL did not show any inhibitory effect on blood biocompatibility of scaffolds when assessed by hemolysis assay. The in vitro cellular behavior was evaluated by growing murine adipose-tissue-derived stem cells (mE-ASCs) over the scaffolds. Enhanced cell proliferation and improved cellular infiltrations on PCL-HS scaffolds were observed when compared to HA containing scaffolds. PCL-HS scaffolds exhibited a significant increase in alkaline phosphatase (ALP) activity and better mineralization of the matrix in comparison to PCL-HA scaffolds. These results clearly demonstrate the stimulatory role of Zn and Si present in HS based composite scaffolds, suggesting their potential application for bone tissue engineering.
本研究旨在比较羟基磷灰石（HA）和硬硅钙石（HS）基复合材料支架的物理化学和生物学特性。硬硅钙石（Ca2ZnSi2O7）粉末通过溶胶-凝胶法合成，而聚己内酯-硬硅钙石（PCL-HS）和聚己内酯-羟基磷灰石（PCL-HA）则通过静电纺丝制成纳米纤维形式。在这两种支架上测定了拉伸强度、细胞增殖、细胞浸润和碱性磷酸酶活性等物理化学和生物学特性。我们发现，与 PCL-HA 支架相比，PCL-HS 支架具有更好的机械强度。溶血试验评估表明，将 HA 和 HS 颗粒添加到 PCL 中不会对支架的血液生物相容性产生任何抑制作用。通过在支架上培养小鼠脂肪组织来源的干细胞（mE-ASCs）来评估体外细胞行为。与含有 HA 的支架相比，PCL-HS 支架上观察到细胞增殖增强和细胞浸润改善。与 PCL-HA 支架相比，PCL-HS 支架的碱性磷酸酶（ALP）活性显著增加，并且基质的矿化更好。这些结果清楚地表明 HS 基复合材料支架中存在的 Zn 和 Si 具有刺激作用，表明它们在骨组织工程中的潜在应用。
