Kitasato Institute for Life Sciences & Graduate School of Infection Control Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Int J Biol Macromol. 2013 Aug;59:192-200. doi: 10.1016/j.ijbiomac.2013.04.054. Epub 2013 Apr 23.
A water-soluble polysaccharide (RP-1) was obtained from distilled rose petals of Rosa damascena Mill. as an attempt for valorization of the waste. RP-1 showed in vitro intestinal immune system modulating activity through Peyer's patch cells and IL-6 producing activity from macrophages. RP-1 lost most of its immunomodulating activity by degradation of the carbohydrate moiety with periodate. RP-1 was fractionated by anion-exchange and gel filtration chromatography and some of the fractions showed significant intestinal immune system modulating activity. The active fractions were suggested to be pectic polysaccharides and type II arabino-3,6-galactan from the component sugar analyses and the reactivity with Yariv antigen. When some active fractions were digested with endo α-d-(1→4)-polygalacturonase, highest molecular weight fragments which were considered as rhamnogalacturonan I, showed potent immunomodulating activities. To our knowledge, this is a first report which explores the possibility for utilization of waste rose petals as a source of immunomodulating pectic polysaccharides.
从蒸馏的大马士革玫瑰花瓣（Rosa damascena Mill.）中获得一种水溶性多糖（RP-1），试图对废物进行增值利用。RP-1 通过派尔集合淋巴结细胞和巨噬细胞产生的 IL-6 显示出体外肠道免疫系统调节活性。RP-1 失去了大部分免疫调节活性，因为其碳水化合物部分被过碘酸盐降解。RP-1 通过阴离子交换和凝胶过滤色谱进行分离，一些馏分显示出显著的肠道免疫系统调节活性。根据成分糖分析和与 Yariv 抗原的反应性，这些活性馏分被认为是果胶多糖和 II 型阿拉伯-3,6-半乳糖聚糖。当一些活性馏分用内切α-d-(1→4)-多聚半乳糖醛酸酶消化时，被认为是鼠李半乳糖醛酸 I 的最高分子量片段显示出强大的免疫调节活性。据我们所知，这是第一个探索利用废玫瑰花瓣作为免疫调节果胶多糖来源的可能性的报告。
