Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China.
Molecules. 2022 Jul 8;27(14):4379. doi: 10.3390/molecules27144379.
is widely used in the essential oil industry and generates large amounts of waste annually. The purpose of this research is the recycling of bioactive flavonoids from rose waste biomass to develop high-value products. Resin screening and adsorption/desorption dynamic analysis showed that HP20 resin was suitable to purify the flavonoids from waste extracts. Under the optimal enrichment process, the product had a 10.7-fold higher purity of flavonoids with a satisfactory recovery of 82.02%. In total, 14 flavonoids were identified in the sample after purification by UHPLC-QTOF-MS. Moreover, the DPPH and ABTS assays revealed that the flavonoids-purified extracts exhibited higher antioxidant activities than the crude extracts. Meanwhile, the purified extracts presented stronger antiproliferative activity against HepG2, Caco-2, MCF-7 and A549 cell lines. The bacteriostatic effects of the purified extracts against four bacteria (, , , )) and yeast ()) were stronger compared with the crude extracts. It was concluded that flavonoids-enriched extracts from waste had the potential to be applied in functional food and pharmaceutical industries.
玫瑰废弃物中的生物活性类黄酮的回收与纯化及其抗氧化和细胞毒活性评价
玫瑰废弃物在精油行业中被广泛应用，每年会产生大量的废弃物。本研究旨在从玫瑰废弃物中回收生物活性类黄酮，以开发高附加值产品。树脂筛选和吸附/解吸动态分析表明，HP20 树脂适合从玫瑰废弃物提取物中纯化类黄酮。在最佳富集工艺条件下，得到的产品中类黄酮的纯度提高了 10.7 倍，回收率为 82.02%。经 UHPLC-QTOF-MS 纯化后，共鉴定出 14 种类黄酮。此外，DPPH 和 ABTS 测定结果表明，纯化提取物的抗氧化活性高于粗提物。同时，纯化提取物对 HepG2、Caco-2、MCF-7 和 A549 细胞系的增殖抑制活性也强于粗提物。与粗提物相比，纯化提取物对四种细菌（、、、）和酵母（）的抑菌效果更强。综上所述，玫瑰废弃物中富含类黄酮的提取物具有在功能性食品和制药行业中应用的潜力。
