Gansu High Throughput Screening and Creation Center for Health Products, School of Pharmacy, Lanzhou University, Lanzhou, Gansu 730020, P.R. China.
Int J Mol Med. 2018 Sep;42(3):1411-1417. doi: 10.3892/ijmm.2018.3738. Epub 2018 Jun 21.
Kushui rose (R. Setate x R. Rugosa) (KR) is a traditional Chinese medicine proven to be a potent antioxidant, and used for thousands of years. Approximately 30% of all human cancers relevant to mutational activated Ras, and over-activated Ras are accompanied by increased accumulation of reactive oxygen species (ROS). Thus, one way of developing anticancer drugs is to reduce ROS accumulation. Therefore, KR was predicted to have potential to combat over-activated Ras-related cancer. C. elegans with let‑60(gf)/ras mutant, which exhibited tumor-like symptoms of the multivulva phenotype, was employed to determine the effect of KR on Ras/MAPK pathway. Other strains of worms and H2DCF-DA dye were also applied to study the antioxidant stress capacity of KR. This study was aimed to determine whether KR has a potential effect on combat over-activated Ras-related cancer through resistance to oxidative stress. Our results showed that Kushui rose decoction (KRD) has potent antioxidant activity in vitro, and can inhibit over-activated Ras in vivo. Further, KRD significantly suppressed over-activated Ras/MAPK pathway by regulating oxidative stress-related proteins, such as forkhead transcription factor (DAF-16), glutathione S-transferase-4 (GST-4), superoxide dismutases (SODs) and heat shock protein-16.2 (HSP-16.2). However, essential oil and hydrosol of KR had no effect on over-activated Ras. Thus these results reminded us that people usually soak rose in hot water to prepare 'rose tea' as an effective way for health care. Thus, KRD was demonstrated to be a potential drug candidate for combating over-activated Ras-related cancer as an antioxidant.
翠雀玫瑰（R. Setate × R. Rugosa）（KR）是一种传统的中药，被证明具有很强的抗氧化作用，已经使用了数千年。大约 30%与突变激活 Ras 相关的人类癌症，以及过度激活的 Ras 伴随着活性氧物质（ROS）的积累增加。因此，开发抗癌药物的一种方法是减少 ROS 积累。因此，KR 被预测具有对抗过度激活 Ras 相关癌症的潜力。用具有 let-60（gf）/ras 突变的秀丽隐杆线虫，表现出多阴门表型的肿瘤样症状，来确定 KR 对 Ras/MAPK 通路的影响。还使用了其他品系的线虫和 H2DCF-DA 染料来研究 KR 的抗氧化应激能力。本研究旨在确定 KR 是否通过抵抗氧化应激对对抗过度激活 Ras 相关癌症具有潜在影响。我们的结果表明，翠雀玫瑰汤（KRD）具有很强的体外抗氧化活性，并能抑制体内过度激活的 Ras。此外，KRD 通过调节与氧化应激相关的蛋白质，如叉头转录因子（DAF-16）、谷胱甘肽 S-转移酶-4（GST-4）、超氧化物歧化酶（SODs）和热休克蛋白-16.2（HSP-16.2），显著抑制过度激活的 Ras/MAPK 通路。然而，KR 的精油和水醇提取物对过度激活的 Ras 没有影响。因此，这些结果提醒我们，人们通常将玫瑰浸泡在热水中以制备“玫瑰茶”，作为一种有效的保健方法。因此，KRD 被证明是一种有潜力的药物候选物，可作为抗氧化剂对抗过度激活 Ras 相关癌症。
