Vollmer G, Kniewe M, Meyn U, Tuchel L, Arnholdt H, Knuppen R
Institut für Biochemische Endokrinologie, Lübeck, F.R.G.
J Steroid Biochem. 1990 Jun;36(1-2):43-55. doi: 10.1016/0022-4731(90)90112-6.
The expression of steroid hormone receptors as molecules reflecting processes of development and differentiation in the human uterine tissue was analysed in a spatial distinct fashion: tissue samples were excised at the fundus and at different, spatially distinct positions of the uterus. They were analysed for concentrations of cytosolic estrogen and progesterone receptors in supernatants from frozen sections using an isoelectric focusing technique. The spatial and molecular distinct, qualitative and quantitative pattern of their expression in the human uterus and uterine adenocarcinomas were studied by sectioning tissue sample from the functionalis through the basalis of the endometrium until reaching deep myometrial parts of the tissue: (1) Specific spatial patterns of estrogen and progesterone receptor levels were detectable throughout the menstrual cycle. (2) For proliferative endometrium from the functionalis to the basalis of the endometrium, the content of both cytosolic receptor species increased up to 6-fold. (3) Differences detectable were less pronounced in the myometrial part of the tissue. (4) Differences of steroid receptor concentrations measured in the endometrium at different uterine positions were highest between fundus and corpus of the endometrium. (5) Maximal differences were detectable around ovulation. (6) After secretory transformation of the organ, specific patterns were still detectable, however quantitative differences were less pronounced. (7) Additionally, quantitative differences measurable were accompanied by variations of molecular properties of the progesterone receptor as demonstrated in an isoelectric focusing gel. (8) In endometrial adenocarcinomas, not only significant quantitative alterations in steroid receptor content were measured, but also a significantly changed spatial pattern of receptor concentrations, also a change of the molecular properties of the progesterone receptor was resolved if these tumor parameters were compared to those detected in the normal tissue of the same organ surrounding the tumor.
在子宫底部和子宫不同的、空间上不同的位置切除组织样本。使用等电聚焦技术分析冷冻切片上清液中细胞溶质雌激素和孕激素受体的浓度。通过将来自子宫内膜功能层直至基底层的组织样本切片，直至到达组织的深部肌层部分，研究了它们在人体子宫和子宫腺癌中表达的空间和分子特异性、定性和定量模式：（1）在整个月经周期中可检测到雌激素和孕激素受体水平的特定空间模式。（2）对于从子宫内膜功能层到基底层的增殖期子宫内膜，两种细胞溶质受体种类的含量增加高达6倍。（3）在组织的肌层部分可检测到的差异不太明显。（4）在子宫内膜不同位置测量的甾体受体浓度差异在子宫底部和子宫体之间最高。（5）在排卵前后可检测到最大差异。（6）器官分泌转化后，仍可检测到特定模式，然而定量差异不太明显。（7）此外，可测量的定量差异伴随着孕激素受体分子性质的变化，如在等电聚焦凝胶中所示。（8）在子宫内膜腺癌中，不仅测量到甾体受体含量有显著的定量改变，而且受体浓度的空间模式也有显著改变，如果将这些肿瘤参数与在肿瘤周围同一器官的正常组织中检测到的参数进行比较，还可分辨出孕激素受体分子性质的变化。
