Berchuck A, Soisson A P, Olt G J, Soper J T, Clarke-Pearson D L, Bast R C, McCarty K S
Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC 27710.
Am J Obstet Gynecol. 1989 Nov;161(5):1247-52. doi: 10.1016/0002-9378(89)90676-5.
Epidermal growth factor receptor expression in fresh-frozen uterine tissues was studied with the use of monoclonal antibody 528, which recognizes an epitope on the external domain of the epidermal growth factor receptor. Immunohistochemically detectable epidermal growth factor receptor was seen in all uterine cell types in 19 of 20 normal uteri. Staining of endometrial glands and endometrial stromal cells was consistently greater than that of myometrium, and no variation in intensity or distribution of staining was seen during the menstrual cycle. Immunohistochemically detectable epidermal growth factor receptor was found less frequently in endometrial adenocarcinomas than in normal endometrium (p less than 0.01). Thirteen of 40 endometrial adenocarcinomas (32.5%) did not express detectable receptor. Epidermal growth factor receptor expression did not correlate with histologic grade, depth of myometrial invasion, estrogen-progesterone receptor status, the presence of extrauterine metastases, or the development of recurrent disease.
利用单克隆抗体528对新鲜冷冻子宫组织中的表皮生长因子受体表达进行了研究，该抗体可识别表皮生长因子受体胞外域的一个表位。在20例正常子宫的19例中，所有子宫细胞类型均可见免疫组织化学可检测到的表皮生长因子受体。子宫内膜腺体和子宫内膜间质细胞的染色始终强于子宫肌层，且在月经周期中未见染色强度或分布的变化。与正常子宫内膜相比，子宫内膜腺癌中免疫组织化学可检测到的表皮生长因子受体较少见（p<0.01）。40例子宫内膜腺癌中有13例（32.5%）未表达可检测到的受体。表皮生长因子受体表达与组织学分级、肌层浸润深度、雌激素-孕激素受体状态、宫外转移的存在或复发性疾病的发生无关。
