Department of Life Science and Engineering, Institute of Technology, Harbin 150001, P. R. China.
J Microbiol. 2013 Apr;51(2):174-82. doi: 10.1007/s12275-013-2545-7. Epub 2013 Apr 27.
Trichoderma harzianum 88, a filamentous soil fungus, is an effective biocontrol agent against several plant pathogens. High-throughput sequencing was used here to study the mycoparasitism mechanisms of T. harzianum 88. Plate confrontation tests of T. harzianum 88 against plant pathogens were conducted, and a cDNA library was constructed from T. harzianum 88 mycelia in the presence of plant pathogen cell walls. Randomly selected transcripts from the cDNA library were compared with eukaryotic plant and fungal genomes. Of the 1,386 transcripts sequenced, the most abundant Gene Ontology (GO) classification group was "physiological process". Differential expression of 19 genes was confirmed by real-time RT-PCR at different mycoparasitism stages against plant pathogens. Gene expression analysis revealed the transcription of various genes involved in mycoparasitism of T. harzianum 88. Our study provides helpful insights into the mechanisms of T. harzianum 88-plant pathogen interactions.
哈茨木霉 88，一种丝状土壤真菌，是几种植物病原体的有效生物防治剂。本研究采用高通量测序技术研究哈茨木霉 88 的菌寄生机制。进行了哈茨木霉 88 对抗植物病原体的平板对峙试验，并在存在植物病原体细胞壁的情况下构建了哈茨木霉 88 菌丝 cDNA 文库。随机选择 cDNA 文库中的转录本与真核植物和真菌基因组进行比较。在测序的 1386 个转录本中，丰度最高的基因本体论 (GO) 分类组是“生理过程”。通过实时 RT-PCR 在不同的菌寄生阶段对植物病原体进行检测，确认了 19 个基因的差异表达。基因表达分析揭示了哈茨木霉 88 菌寄生过程中各种基因的转录。本研究为哈茨木霉 88-植物病原体相互作用的机制提供了有价值的见解。
