Universidade Estadual de Goiás, UnUCET, BR 153, Km 98 Campus Henrique Santillo, Anápolis, GO CEP 75000-000, Brazil.
Curr Microbiol. 2010 Oct;61(4):298-305. doi: 10.1007/s00284-010-9611-8. Epub 2010 Mar 7.
Trichoderma harzianum ALL42 were capable of overgrowing and degrading Rhizoctonia solani and Macrophomina phaseolina mycelia, coiling around the hyphae with formation of apressoria and hook-like structures. Hyphae of T. harzianum ALL42 did not show any coiling around Fusarium sp. hyphae suggesting that mycoparasitism may be different among the plant pathogens. In this study, a secretome analysis was used to identify some extracellular proteins secreted by T. harzianum ALL42 after growth on cell wall of M. phaseolina, Fusarium sp., and R. solani. The secreted proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. A total of 60 T. harzianum ALL42 secreted proteins excised from the gel were analyzed from the three growth conditions. While seven cell wall-induced proteins were identified, more than 53 proteins spots remain unidentified, indicating that these proteins are either novel proteins or proteins that have not yet been sequenced. Endochitinase, β-glucosidase, α-mannosidase, acid phosphatase, α-1,3-glucanase, and proteases were identified in the gel and also detected in the supernatant of culture.
哈茨木霉 ALL42 能够覆盖和降解立枯丝核菌和茄腐皮镰孢菌菌丝，形成附着胞和钩状结构缠绕在菌丝周围。哈茨木霉 ALL42 的菌丝不会缠绕在镰刀菌属的菌丝周围，这表明其对植物病原体的寄生作用可能不同。在这项研究中，通过对哈茨木霉 ALL42 在生长于茄腐皮镰孢菌、镰刀菌属和立枯丝核菌细胞壁后的胞外蛋白进行分析，采用分泌组学技术来鉴定其分泌的一些蛋白。用二维电泳和 MALDI-TOF 质谱对分泌蛋白进行分析。从三种生长条件下从凝胶中提取了 60 个哈茨木霉 ALL42 分泌蛋白进行分析。鉴定出了 7 个细胞壁诱导蛋白，但仍有超过 53 个蛋白斑点未被鉴定，表明这些蛋白要么是新的蛋白，要么是尚未测序的蛋白。在凝胶中鉴定到了几丁质酶、β-葡萄糖苷酶、α-甘露糖苷酶、酸性磷酸酶、α-1,3-葡聚糖酶和蛋白酶，这些酶在培养物上清液中也有检测到。
