Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
Fermentation and Functionality Research Group, Korea Food Research Institute, Sungnam 463-746, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3640-3645. doi: 10.1099/ijs.0.048116-0. Epub 2013 Apr 26.
A Gram-staining-negative, strictly aerobic, rod-shaped bacterium, designated CBA3202(T), was isolated from seashore sand on Jeju Island, Republic of Korea. Based on the 16S rRNA gene sequence analysis, strain CBA3202(T) was allocated to the genus Gillisia (family Flavobacteriaceae) and was most closely related to the type strain of Gillisia mitskevichiae (99.0 % 16S rRNA gene sequence similarity). Optimal growth occurred at 25 °C and with 3 % NaCl. The only isoprenoid quinone was menaquinone-6 (MK-6), the predominant fatty acids were C16 : 0, iso-C15 : 1 G, iso-C16 : 0 and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), and the DNA G+C content was 34.9 mol%. The polar lipids were phosphatidylethanolamine, two unidentified aminolipids and several unidentified polar lipids. Based on phylogenetic inference and phenotypic data, we conclude that strain CBA3202(T) represents a novel species of the genus Gillisia, for which the name Gillisia marina sp. nov. is proposed. The type strain is CBA3202(T) ( = KACC 16693(T) = KCTC 32030(T) = JCM 18402(T)). An emended description of the genus Gillisia is also provided.
一株革兰氏阴性、严格需氧、杆状的细菌，被命名为 CBA3202(T)，分离自韩国济州岛的海滨沙滩。基于 16S rRNA 基因序列分析，菌株 CBA3202(T)被分配到 Gillisia 属（Flavobacteriaceae 科），与 Gillisia mitskevichiae 的模式菌株最为密切相关（99.0% 16S rRNA 基因序列相似性）。最佳生长条件为 25°C 和 3%NaCl。唯一的类异戊二烯醌是menaquinone-6 (MK-6)，主要脂肪酸是 C16:0、iso-C15:1 G、iso-C16:0 和总和特征 3（包括 C16:1ω6c 和/或 C16:1ω7c），DNA G+C 含量为 34.9 mol%。极性脂包括磷脂酰乙醇胺、两种未鉴定的胺脂和几种未鉴定的极性脂。基于系统发育推断和表型数据，我们得出结论，菌株 CBA3202(T)代表 Gillisia 属的一个新种，命名为 Gillisia marina sp. nov.。模式菌株为 CBA3202(T)（=KACC 16693(T)=KCTC 32030(T)=JCM 18402(T)）。还提供了 Gillisia 属的修订描述。
