Department of Analytical Chemistry, Faculty of Chemical Engineering, Institute of Chemical Technology, Prague, Czech Republic.
J Sep Sci. 2013 Jul;36(13):2072-80. doi: 10.1002/jssc.201300116. Epub 2013 Jun 3.
We prepared new phases for LC that consisted of silica modified with non-covalently bonded tetrakis(β-cyclodextrin)-porphyrin (where cyclodextrin is CD) conjugates. The effects of the porphyrin core, type of spacer and β-CD moieties on the behaviours of the modified phases for the separation of aromatic compounds (benzene, toluene, ethylbenzene, propylbenzene, butylbenzene, pentylbenzene, o-terphenyl, triphenylene, phenol and caffeine) and fluorinated aromatic compounds (pentafluorobenzonitrile, pentafluoronitrobenzene and hexafluorobenzene) were studied using the Tanaka test. The results indicate that the non-covalent substitution of silica with CD-based macromolecules that have a porphyrin core can be a very effective method for preparing novel sorbents with specific chromatographic properties for applications in LC.
我们为 LC 准备了新的固定相，这些固定相由非共价键合的四（β-环糊精）-卟啉（其中环糊精为 CD）偶联物修饰的硅胶组成。研究了卟啉核心、间隔基类型和β-CD 部分对改性固定相分离芳香族化合物（苯、甲苯、乙苯、丙苯、丁苯、戊苯、邻三联苯、三联苯、苯酚和咖啡因）和氟化芳香族化合物（五氟苯甲腈、五氟硝基苯和六氟苯）的行为的影响，使用了 Tanaka 测试。结果表明，硅胶与具有卟啉核心的基于 CD 的大分子的非共价取代可以是一种非常有效的方法，用于制备具有特定色谱性能的新型吸附剂，用于 LC 中的应用。
