Iovanovich B, Nechipurenko Iu D
Mol Biol (Mosk). 1990 Mar-Apr;24(2):478-86.
The effect of finite fragment length on the distribution pattern of bound protein along the DNA fragment is considered. If the size of the binding site for a ligand on DNA is comparable with the length of the DNA fragment fluctuations in the amount of ligand bound to the fragment create some difficulties for evaluating the distribution pattern of ligand on DNA. A mathematical approach is developed which enables one to calculate the distribution pattern of ligand on DNA provided that the number of bound ligand on the DNA fragment is known. Expression are also obtained to treat the effects of fluctuations in the number of ligand molecules bound to the DNA fragment on the distribution pattern of ligand. A new procedure is proposed which may be useful for locating the preferable binding sites for ligand on DNA on the basis of footprinting experiments.
考虑了有限片段长度对结合蛋白沿DNA片段分布模式的影响。如果DNA上配体结合位点的大小与DNA片段长度相当，那么与片段结合的配体数量的波动会给评估配体在DNA上的分布模式带来一些困难。本文开发了一种数学方法，只要知道DNA片段上结合配体的数量，就能计算出配体在DNA上的分布模式。还获得了一些表达式，用于处理与DNA片段结合的配体分子数量波动对配体分布模式的影响。提出了一种新方法，该方法可能有助于根据足迹实验确定配体在DNA上的优先结合位点。
