Zhang Cui-Ying, Wang Jie, Guo Li-Li, Cheng Hui-Ping, Cui Han-Ming, Wu Ping, Dong Yu, Huang Shi-Jing
Guang'anmen hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
Zhong Yao Cai. 2012 Nov;35(11):1758-62.
To establish an HPLC method of a characteristical chemical fingerprint analysis in combination with simultaneous determination of four bioactive components for species differentiation and quality assessment of Ziziphus jujuba var. spinosa.
The chromatographic separation was performed on an Agilent TC-C18 BDS (250 mm x 4.6 mm, 5 microm) column. The mobile phase consisted of acetonitrile and water in a linear gradient elution procedure. The evaporator tube temperature of ELSD was set at 110.5 degrees C with the nebulizing gas flow rate of 3.1 mL/min and the flow rate of mobile phase was 1.0 mL/min. The column was maintained at 30 degrees C. The injection volume was 20 microL.
HPLC methodology for both chemical fingerprint analysis and quantitative determination of four active ingredients were validated, respectively. According to the contents of the four ingredients and the chemical fingerprints of Ziziphus jujuba var. spinosa using principal component analysis, Ziziphus jujuba var. spinosa was different from the fake derived from the seeds of Ziziphus mauritiana.
The developed HPLC method is exclusive and repetitive for the species identification and quality evaluation of Ziziphus jujuba var. spinosa.
建立一种高效液相色谱法，用于酸枣仁特征性化学指纹图谱分析，并同时测定四种生物活性成分，以进行酸枣仁的品种鉴别和质量评价。
采用安捷伦TC-C18 BDS（250 mm×4.6 mm，5 µm）色谱柱进行色谱分离。流动相由乙腈和水组成，采用线性梯度洗脱程序。蒸发光散射检测器（ELSD）的蒸发管温度设定为110.5℃，雾化气体流速为3.1 mL/min，流动相流速为1.0 mL/min。柱温保持在30℃。进样量为20 µL。
分别对化学指纹图谱分析和四种活性成分定量测定的高效液相色谱方法进行了验证。根据四种成分的含量和酸枣仁的化学指纹图谱，采用主成分分析法，酸枣仁与源自毛叶枣种子的伪品不同。
所建立的高效液相色谱法对酸枣仁的品种鉴定和质量评价具有专属性和重复性。
