Chen Yan-Fen, Wang Chun-Yi, Li Wei-Min, Gao Ying, Wu Qing-He, Tang Chun-Ping, Wang Hui, Yang Chao-Yan
School of Traditional Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhong Yao Cai. 2012 Nov;35(11):1809-13.
To study the effect of HGD on diabetic cardiomyopathy and its mechanism.
The T2-DM rats model was established by combining high fat diet with STZ. The blood glucose, insulin, myocardial fibrosis and TGF-beta1/Smad3 signaling pathway were observed; TGF-beta1 and Smad3 mRNA expression were detected by RT-PCR method, protein expression detected by immunohistochemical method.
HGD obviously reduced fasting blood glucose, insulin, improved insulin resistance, reduced myocardial hydroxyproline contents, lowered cardiac index, significantly inhibited over-expression of TGF-beta1/SMAD3 mRNA and protein in diabetic rats cardiac.
HGD can obviously prevent experimental diabetic myocardial fibrosis through the regulation effect on TGFbeta1/Smad3 signaling pathway.
研究HGD对糖尿病心肌病的影响及其机制。
采用高脂饮食联合链脲佐菌素建立T2-DM大鼠模型。观察血糖、胰岛素、心肌纤维化及TGF-β1/Smad3信号通路；采用RT-PCR法检测TGF-β1和Smad3 mRNA表达，免疫组化法检测蛋白表达。
HGD明显降低空腹血糖、胰岛素，改善胰岛素抵抗，降低心肌羟脯氨酸含量，降低心脏指数，显著抑制糖尿病大鼠心脏中TGF-β1/SMAD3 mRNA和蛋白的过度表达。
HGD可通过对TGFβ1/Smad3信号通路的调节作用明显预防实验性糖尿病心肌纤维化。
