The Endocrinology Department of Guang'anmen Hospital of China Academy of Chinese Medical Sciences, Beijing 100053, China.
J Tradit Chin Med. 2011 Sep;31(3):209-19. doi: 10.1016/s0254-6272(11)60044-7.
To study the effect of Shengmai San ((see text) Pulse-activating Powder) in protecting myocardium in the rat of the type 2 diabetic cardiomyopathy (DCM) model.
The DCM rat model was established by combination of insulin resistance induced by a high-fat diet with intraperitoneal injection of high dose streptozotocin (50 mg/kg). And these rat models were randomly divided into three groups: a normal group (n = 12,one of them died), a model group (n = 15) and a Shengmai San group (treatment group, n = 15).The damage of the myocardium was assessed by electrocardiogram at the twelfth week after modeling, and the blood glucose, cholesterol and triglyceride levels were determined; the content of the left cardiac ventricle myocardial collagen was quantified by Masson staining test; the level of myocardial cell apoptosis was detected with TUNEL apoptosis detection kit; the damage extent of the myocardial sub-cellular structures was observed by electron microscopy; the expression levels of cardiac TSP-1 (Thrombospondin-1), TGF-beta1 (Transforming Growth F factor-beta) and TRB-3 (Tribbles homolog 3) proteins were detected by immunohistochemical method; the expression levels of cardiac TSP-1, A-TGF-beta1 and L-TGF-beta1 proteins were detected by Western blotting; and the expression levels of TSP-1 and TRB-3 mRNAs were detected by real-time quantitative PCR.
Compared with the control group, the blood glucose, cholesterol, triglycerides levels in both the model groups and the Shengmai San group were significantly decreased; the myocardial tissue was less damaged and the collagen content was reduced in the Shengmai San group; the myocardial sub-cellular structure was injured to a lesser extent; the expression levels of myocardial TSP-1, TGF-beta1, TRB-3, and TSP-1, A-TGF-beta1, L-TGF-beta1 and chymase were decreased, and the expression levels of TSP-1 mRNA and TRB-3 mRNA were decreased in both the model groups and the Shengmai San group (the latter was better),.
Shengmai San can inhibit myocardial fibrosis in the rat of diabetic cardiomyopathy, and significantly delay the formation of diabetic cardiomyopathy in hyperglycemia rats through multiple pathways.
研究生脉散（见正文）对 2 型糖尿病心肌病（DCM）模型大鼠心肌的保护作用。
采用高脂饮食联合腹腔注射大剂量链脲佐菌素（50mg/kg）建立 DCM 大鼠模型。将这些大鼠模型随机分为三组：正常组（n=12，其中 1 只死亡）、模型组（n=15）和生脉散组（治疗组，n=15）。在建模后第 12 周，通过心电图评估心肌损伤，测定血糖、胆固醇和甘油三酯水平；采用 Masson 染色法检测左心室心肌胶原含量；TUNEL 凋亡检测试剂盒检测心肌细胞凋亡水平；电镜观察心肌亚细胞结构损伤程度；免疫组织化学法检测心脏 TSP-1（血小板反应蛋白-1）、TGF-β1（转化生长因子-β）和 TRB-3（Tribbles 同源物 3）蛋白的表达水平；Western 印迹法检测心脏 TSP-1、A-TGF-β1 和 L-TGF-β1 蛋白的表达水平；实时定量 PCR 法检测 TSP-1 和 TRB-3 mRNA 的表达水平。
与对照组相比，模型组和生脉散组的血糖、胆固醇、甘油三酯水平均显著降低；生脉散组心肌组织损伤减轻，胶原含量减少；心肌亚细胞结构损伤程度较轻；心肌 TSP-1、TGF-β1、TRB-3 及 TSP-1、A-TGF-β1、L-TGF-β1 和糜酶的表达水平降低，模型组和生脉散组 TSP-1mRNA 和 TRB-3mRNA 的表达水平降低（后者较好）。
生脉散可抑制糖尿病心肌病大鼠心肌纤维化，通过多种途径显著延缓高血糖大鼠糖尿病心肌病的形成。
