[从蛇足石杉中筛选具有乙酰胆碱酯酶抑制活性的内生真菌及其分类鉴定]
[Screening of endophytic fungi from Huperzia serrata for acetylcholinesterase inhibitory activity and its taxonomic identification].
作者信息
Wang Li-Li, Lv Hui-Fang, Zhang Li, Hua Hai-Xia, Wang Jie-Hua, Hu Zhi-Bi, Li Wan-Kui
机构信息
The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(24):3701-5.
OBJECTIVE
To screen out fungus strains with acetylcholinesterase inhibitory activity from Huperzia serrata.
METHOD
Endophytic fungi fermentation products from 59 H. serrata strains were stained with acetylcholinesterase hydrolyzed alpha-naphthaleneacetic ethyl ester and fast blue B salt, and screened for acetylcholinesterase inhibitory activity with thin-layer chromatography-bioautography. Target strains were classified and identified through the sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics.
RESULT
Fungus strain LQ2F01 from H. serrata showed positive color reaction in the screening for acetylcholinesterase inhibitory activity. The sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics showed the strain LQ2F01 belonged to Acremonium.
CONCLUSION
Endophytic Fungi LQ2F01 from H. serrata shows identical acetylcholinesterase inhibitory activity with the host plant, which is of great significance to the development of natural medicines and the studies on the relationship between the endophytic gungi and the host plant.
目的
从蛇足石杉中筛选出具有乙酰胆碱酯酶抑制活性的真菌菌株。
方法
用乙酰胆碱酯酶水解α-萘乙酸乙酯和固蓝B盐对59株蛇足石杉内生真菌发酵产物进行染色，采用薄层色谱-生物自显影法筛选乙酰胆碱酯酶抑制活性。通过对18s rDNA和5.8s rDNA进行序列分析并结合形态特征对目标菌株进行分类鉴定。
结果
蛇足石杉真菌菌株LQ2F01在乙酰胆碱酯酶抑制活性筛选中呈阳性显色反应。对18s rDNA和5.8s rDNA进行序列分析并结合形态特征表明菌株LQ2F01属于枝顶孢属。
结论
蛇足石杉内生真菌LQ2F01与宿主植物具有相同的乙酰胆碱酯酶抑制活性，这对天然药物的开发以及内生真菌与宿主植物关系的研究具有重要意义。
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