Zhejiang Pharmaceutical College, Yinxian Road 888, Yinzhou District, Ningbo, 315100, China,
World J Microbiol Biotechnol. 2014 Mar;30(3):1011-7. doi: 10.1007/s11274-013-1519-6. Epub 2013 Oct 16.
This present study was designed to investigate the production of huperzine A (HupA), an acetylcholine inhibitor, which was produced by an endophytic fungi isolated from Huperzia serrata. Screening of 94 endophytic fungal isolates obtained from plant H. serrata was carried out for the production of HupA. Their morphological characteristics were studied and rDNA sequence analysis was carried out. The cultures were grown in liquid culture medium and the extracted metabolites were analyzed by thin layer chromatography and high performance liquid chromatograph for the presence of HupA. The DPPH scavenging ratio and inhibition ratio of acetylcholinesterase (AchE) of the same were determined. 3 out of 94 strains i.e. S29, L44 and S94 showed significant AchE-inhibitory activity and antioxidant activity. Strain L44 which exhibited maximum yield of HupA (37.63 μg/g on dry weight basis) was identified as Trichoderma species by ITS sequence analysis. In conclusion, endophytic fungi from H. serrata can be used as a new resource of HupA.
本研究旨在探索从蛇足石杉中分离得到的内生真菌产生的石杉碱甲（HupA），这是一种乙酰胆碱抑制剂。对从植物蛇足石杉中分离得到的 94 株内生真菌进行筛选，以生产 HupA。研究了它们的形态特征，并进行了 rDNA 序列分析。在液体培养基中培养这些菌株，并通过薄层层析和高效液相色谱法分析提取的代谢产物，以确定是否存在 HupA。还测定了它们的 DPPH 清除率和乙酰胆碱酯酶（AchE）抑制率。在 94 株菌株中，有 3 株即 S29、L44 和 S94 表现出显著的 AchE 抑制活性和抗氧化活性。其中菌株 L44 产生的 HupA 产量最高（干重基础上为 37.63μg/g），通过 ITS 序列分析鉴定为木霉属。总之，蛇足石杉中的内生真菌可以作为 HupA 的新资源。
