Qi Bo-Wen, Mo Ting, Zhang Xin, Yan Ya-Ru, Xu Xi-Ping, Yang Hong-Yun, Wang Xiao-Hui, Li Jun, Shi She-Po, Liu Xiao
Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
Ningxia Hui Medicine Research Institute Yinchuan 750021,China.
Zhongguo Zhong Yao Za Zhi. 2019 Aug;44(15):3213-3220. doi: 10.19540/j.cnki.cjcmm.20190527.101.
A total of 27 endophytic fungal strains were isolated from Huperzia serrata,which were richly distributed in the stems and leaves while less distributed in roots. The 27 strains were identified by Internal Transcribed Spacer( ITS) r DNA molecular method and one of the strains belongs to Basidiomycota phylum,and other 26 stains belong to 26 species,9 general,6 families,5 orders,3 classes of Ascomycota Phylum. The dominant strains were Colletotrichum genus,belonging to Glomerellaceae family,Glomerellales order,Sordariomycetes class,Ascomycota Phylum,with the percentage of 48. 15%. The inhibitory activities of the crude extracts of 27 endophytic fungal strains against acetylcholinesterase( ACh E) and nitric oxide( NO) production were evaluated by Ellman's method and Griess method,respectively. Crude extracts of four fungi exhibited inhibitory activities against ACh E with an IC50 value of 42. 5-62. 4 mg·L(-1),and some fungi's crude extracts were found to inhibit nitric oxide( NO) production in lipopolysaccharide( LPS)-activated RAW264. 7 macrophage cells with an IC50 value of 2. 2-51. 3 mg·L(-1),which indicated that these fungi had potential anti-inflammatory activities.The chemical composition of the Et OAc extract of endophytic fungus HS21 was also analyzed by LCMS-IT-TOF. Seventeen compounds including six polyketides,four diphenyl ether derivatives and seven meroterpenoids were putatively identified.
从蛇足石杉中总共分离出27株内生真菌菌株，它们在茎和叶中分布丰富，而在根中分布较少。通过内转录间隔区（ITS）rDNA分子方法对这27株菌株进行了鉴定，其中1株属于担子菌门，其他26株属于子囊菌门的26个种、9个属、6个科、5个目、3个纲。优势菌株为炭疽菌属，属于子囊菌门粪壳菌纲炭角菌目小丛壳科，占比48.15%。分别采用Ellman法和Griess法评估了27株内生真菌菌株粗提物对乙酰胆碱酯酶（AChE）和一氧化氮（NO）产生的抑制活性。4株真菌的粗提物对AChE表现出抑制活性，IC50值为42.5 - 62.4 mg·L⁻¹，并且发现一些真菌的粗提物能够抑制脂多糖（LPS）激活的RAW264.7巨噬细胞中一氧化氮（NO）的产生，IC50值为2.2 - 51.3 mg·L⁻¹，这表明这些真菌具有潜在的抗炎活性。还通过LCMS - IT - TOF分析了内生真菌HS21的乙酸乙酯提取物的化学成分。初步鉴定出17种化合物，包括6种聚酮化合物、4种二苯醚衍生物和7种半萜类化合物。
