a School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou , China.
Nat Prod Res. 2013;27(22):2157-60. doi: 10.1080/14786419.2013.790031. Epub 2013 Apr 29.
Five taxane diterpenoids derived from the 95% ethanol extract of Taxus chinensis were tested for the inhibitory activities on amyloid-beta (Aβ) peptide aggregation. Using thioflavin-T fluorescence assay, sciadopitysin was found to exhibit the most potency against Aβ aggregation and the formation of fibrils. Further cellular assay indicated that sciadopitysin increased the cell viability of SH-SY5Y cell and demonstrated neuroprotection against Aβ protein-induced insult in primary cortical neurons. According to the authors' best knowledge, this is the first report that sciadopitysin can inhibit the Aβ aggregation and reduce Aβ-induced toxicity in the primary cortical neurons.
从中国红豆杉的 95%乙醇提取物中分离得到的 5 种紫杉烷二萜化合物，对淀粉样蛋白-β（Aβ）肽聚集的抑制活性进行了测试。用噻唑蓝荧光法检测，发现船形乌头碱对 Aβ 聚集和纤维形成的抑制活性最强。进一步的细胞试验表明，船形乌头碱可提高 SH-SY5Y 细胞的活力，并对原代皮质神经元中 Aβ 蛋白诱导的损伤表现出神经保护作用。据作者所知，这是船形乌头碱能抑制 Aβ 聚集并降低原代皮质神经元中 Aβ 诱导毒性的首次报道。
