Department of Applied Science, Hong Kong Institute of Vocational Education (Shatin), Shatin, Hong Kong, China.
Pharm Biol. 2013 Jul;51(7):851-5. doi: 10.3109/13880209.2013.767839. Epub 2013 Apr 29.
Scientific evidence has shown Coriolus versicolor (L. ex Fr.) Quel (also known as Yunzhi) has the role of immunomodulator in therapeutic effect. The aim of this in vitro study was to investigate the antioxidative effect of Yunzhi and to explore the mechanisms behind its DNA protection.
Commercial Yunzhi extract was dissolved in water and diluted in five concentrations (10(1)-10(5) μg/L) with appropriate buffers. Lymphocytes harvested from three healthy subjects were incubated with Yunzhi extract for 30 min. Cells were then subjected to 5 min oxidant challenge by 45 μM hydrogen peroxide. The standard alkaline comet (SAC) assay and lysed cell comet (LCC) assay were performed in parallel. DNA damage of each treatment was scored under a fluorescence microscope and compared with the cells without Yunzhi pretreatment.
U-shaped dose-response was seen in both versions of the comet assay. Yunzhi at 10(4) μg/L demonstrated a genoprotective effect against oxidative damage in the SAC assay (25% decrease in comet score). In the LCC assay, a trend of protection in lymphocytes was observed but it did not reach statistical significance.
A direct antioxidant effect of Yunzhi against oxidant challenge on the DNA of lymphocytes was evidenced. The active component in Yunzhi was likely to be membrane permeable.
科学证据表明，云芝（Coriolus versicolor（L. ex Fr.） Quel）具有免疫调节剂的治疗作用。本体外研究旨在探讨云芝的抗氧化作用，并探索其 DNA 保护机制。
将市售云芝提取物溶于水，用适当的缓冲液稀释成五个浓度（10（1）-10（5）μg/L）。从三名健康受试者中采集淋巴细胞，用云芝提取物孵育 30 分钟。然后，将细胞用 45μM 过氧化氢进行 5 分钟的氧化剂挑战。同时进行标准碱性彗星（SAC）试验和裂解细胞彗星（LCC）试验。在荧光显微镜下对每个处理的 DNA 损伤进行评分，并与未经云芝预处理的细胞进行比较。
两种彗星试验均呈现出 U 形剂量反应。SAC 试验中，云芝在 10（4）μg/L 时对氧化损伤表现出保护作用（彗星评分降低 25%）。在 LCC 试验中，观察到淋巴细胞有保护趋势，但未达到统计学意义。
云芝对淋巴细胞 DNA 受到氧化剂挑战具有直接的抗氧化作用。云芝中的活性成分可能是膜通透性的。
