Women's Reproductive Health Laboratory of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
BMC Cancer. 2013 Apr 30;13:216. doi: 10.1186/1471-2407-13-216.
To assess the feasibility of validating microRNA (miRNA) profile related to paclitaxel-sensitivity in formalin-fixed paraffin-embedded (FFPE) samples of serous ovarian carcinoma (OC) patients.
Deregulated miRNAs identified by miRNA microarray were further detected in 45 FFPE OC samples using Realtime PCR. Correlations between paired FFPE and frozen tumor samples were analyzed. Survival times were compared between 6 high and low miRNAs groups. Western blot and luciferase reporter assay were used for validating the target of miRNA.
Sixteen up-regulated miRNAs and twenty-three down-regulated miRNAs were revealed in pacilitaxel-resistant ST30 cells. The up-regulated miRNAs (miR-320a, 22 and 129-5p) and down-regulated miRNAs (miR-9, 155 and 640) were confirmed in paclitaxel-resistant FFPE tumor samples, compared with paclitaxel-sensitive samples. Higher miR-9 and miR-640 showed better survival time in OC patients. Expressions of miR-9, 155 and 22 in FFPE samples were closely mimicked by those in frozen tissues. RAB34 was validated as a direct target of miR-9.
We validated miRNA profile in pacilitaxel-resistant OC using FFPE samples, which might enable treatment stratification and help us to predict outcomes in OC patients. FFPE samples are feasible materials for miRNA research.
评估在福尔马林固定石蜡包埋（FFPE）浆液性卵巢癌（OC）患者样本中验证与紫杉醇敏感性相关的 microRNA（miRNA）谱的可行性。
使用 Realtime PCR 在 45 个 FFPE OC 样本中进一步检测 miRNA 微阵列鉴定的失调 miRNA。分析配对 FFPE 和冷冻肿瘤样本之间的相关性。比较 6 个高和低 miRNA 组之间的生存时间。使用 Western blot 和荧光素酶报告基因测定验证 miRNA 的靶标。
在紫杉醇耐药 ST30 细胞中发现了 16 个上调的 miRNA 和 23 个下调的 miRNA。与紫杉醇敏感样本相比，紫杉醇耐药 FFPE 肿瘤样本中证实了上调的 miRNA（miR-320a、22 和 129-5p）和下调的 miRNA（miR-9、155 和 640）。OC 患者中 miR-9 和 miR-640 表达水平较高，生存时间较好。FFPE 样本中的 miR-9、155 和 22 表达与冷冻组织中的表达密切模拟。RAB34 被验证为 miR-9 的直接靶标。
我们使用 FFPE 样本验证了紫杉醇耐药 OC 中的 miRNA 谱，这可能使治疗分层并帮助我们预测 OC 患者的结局。FFPE 样本是 miRNA 研究的可行材料。
