Seras-Franzoso Joaquin, Tsimbouri Penelope M, Burgess Karl V, Unzueta Ugutz, Garcia-Fruitos Elena, Vazquez Esther, Villaverde Antonio, Dalby Matthew J
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, 08193, Spain.
Nanomedicine (Lond). 2014 Feb;9(2):207-20. doi: 10.2217/nnm.13.43. Epub 2013 Apr 30.
Bacterial inclusion bodies (IBs) are nanostructured (submicron), pseudospherical proteinaceous particles produced in recombinant bacteria resulting from ordered protein aggregation. Being mechanically stable, several physicochemical and biological properties of IBs can be tuned by appropriate selection of the producer strain and of culture conditions. It has been previously shown that IBs favor cell adhesion and surface colonization by mammalian cell lines upon decoration on materials surfaces, but how these biomaterials could influence the behavior of mesenchymal stem cells remains to be explored.
MATERIALS & METHODS: Here, the authors vary topography, stiffness and wettability using the IBs to decorate polycaprolactone surfaces on which mesenchymal stem cells are cultured.
The authors show that these topographies can be used to specifically target osteogenesis from mesenchymal stem cells, and through metabolomics, they show that the cells have increased energy demand during this bone-related differentiation.
IBs as topographies can be used not only to direct cell proliferation but also to target differentiation of mesenchymal stem cells.
细菌包涵体（IBs）是纳米结构（亚微米级）的假球形蛋白质颗粒，由重组细菌中有序的蛋白质聚集产生。由于具有机械稳定性，通过适当选择生产菌株和培养条件，可以调节IBs的几种物理化学和生物学特性。先前已经表明，在材料表面修饰后，IBs有利于哺乳动物细胞系的细胞粘附和表面定植，但这些生物材料如何影响间充质干细胞的行为仍有待探索。
在此，作者使用IBs修饰聚己内酯表面，改变其拓扑结构、硬度和润湿性，然后在该表面培养间充质干细胞。
作者表明，这些拓扑结构可用于特异性地靶向间充质干细胞的成骨作用，并且通过代谢组学，他们表明细胞在这种与骨相关的分化过程中能量需求增加。
作为拓扑结构的IBs不仅可用于指导细胞增殖，还可用于靶向间充质干细胞的分化。
