Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Curr Opin Struct Biol. 2013 Apr;23(2):292-301. doi: 10.1016/j.sbi.2013.04.002. Epub 2013 Apr 27.
Ca(2+)/calmodulin dependent protein kinase II (CaMKII) is a broadly distributed metazoan Ser/Thr protein kinase that is important in neuronal and cardiac signaling. CaMKII forms oligomeric assemblies, typically dodecameric, in which the calcium-responsive kinase domains are organized around a central hub. We review the results of crystallographic analyses of CaMKII, including the recently determined structure of a full-length and autoinhibited form of the holoenzyme. These structures, when combined with other data, allow informed speculation about how CaMKII escapes calcium-dependence when calcium spikes exceed threshold frequencies.
钙/钙调蛋白依赖性蛋白激酶 II（CaMKII）是一种广泛分布的后生动物丝氨酸/苏氨酸蛋白激酶，在神经元和心脏信号转导中很重要。CaMKII 形成寡聚体组装体，通常为十二聚体，其中钙反应性激酶结构域围绕中央枢纽组织。我们综述了 CaMKII 的晶体学分析结果，包括最近确定的全酶的全长和自动抑制形式的结构。这些结构与其他数据结合在一起，可以推测 CaMKII 在钙峰超过阈值频率时如何摆脱钙依赖性。
