Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA.
Opt Lett. 2013 May 1;38(9):1515-7. doi: 10.1364/OL.38.001515.
Fluorescence lifetime imaging (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. There has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed endoscopy systems. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of endoscopy systems that are suitable for in vivo tissue imaging. We present a compact wide-field time-gated FLIM flexible endoscope capable of continuous lifetime imaging of up to three fluorescence emission bands simultaneously. This endoscope design will facilitate the evaluation of FLIM for in vivo applications.
荧光寿命成像（FLIM）为生物组织的生化成分特征提供了一种非侵入性的方法。多光谱 FLIM 在医学诊断中的应用越来越受到关注。FLIM 技术临床转化的核心是开发紧凑型高速内窥镜系统。不幸的是，主要的多光谱 FLIM 方法受到限制，阻碍了适合体内组织成像的内窥镜系统的发展。我们提出了一种紧凑的宽场时间门控 FLIM 柔性内窥镜，能够连续同时对多达三个荧光发射带进行寿命成像。这种内窥镜设计将有助于评估 FLIM 在体内应用中的效果。
