Zhou Rong-Miao, Li Yan, Wang Na, Niu Chao-Xu, Huang Xi, Cao Shi-Ru, Huo Xiang-Ran
Hebei Provincial Cancer Institute, The Fourth Hospital of Hebei Medical University, Hebei Province, China.
Department of Surgery, Shijiazhuang Ping'an Hospital, Hebei Province, China.
Asian Pac J Cancer Prev. 2020 Oct 1;21(10):2987-2992. doi: 10.31557/APJCP.2020.21.10.2987.
Poly (ADP-ribose) polymerase 1 (PARP1), as a key enzyme in the base excision repair pathway, plays a crucial role in tumorigenesis and progression. This study aimed to assess whether polymorphisms of PARP1 gene could be used as predictive biomarkers for the survival of esophageal squamous cell carcinoma (ESCC) patients from Cixian high-incidence region in northern China.
In 203 ESCC patients with survival information, PARP1 rs1136410 T/C and rs8679 T/C single nucleotide polymorphisms (SNPs) were genotyped by polymerase chain reaction ligase detection reaction (PCR-LDR) method. All statistical analyses were performed using the SPSS ver. 22.0 software package (SPSS, Chicago, IL, USA).
The mean age ± standard deviation of the ESCC patients was 60.4 ± 7.9 years. There was no significant relation of sex, age, smoking status and upper gastrointestinal cancer family history with the survival time of the ESCC patients. The mean survival time of rs1136410 T/T, T/C and C/C genotype carriers were 43.3, 42.3 and 46.6 months, respectively. The rs1136410 was not associated with the survival time of the ESCC patients. For rs8679, the mean survival time of T/T genotype carriers was 43.7 months, which was not significantly different from that of the patients with T/C genotype (42.1 months).
In Cixian high-incidence region from northern China, rs1136410 and rs8679 SNPs might not be used to predict survival of ESCC patients. There is a need to explore whether other SNPs of PARP1 gene have an effect on prognosis of ESCC patients.
聚（ADP - 核糖）聚合酶1（PARP1）作为碱基切除修复途径中的关键酶，在肿瘤发生和发展中起关键作用。本研究旨在评估PARP1基因多态性是否可作为中国北方磁县高发地区食管鳞状细胞癌（ESCC）患者生存的预测生物标志物。
对203例有生存信息的ESCC患者，采用聚合酶链反应连接酶检测反应（PCR - LDR）方法对PARP1 rs1136410 T/C和rs8679 T/C单核苷酸多态性（SNP）进行基因分型。所有统计分析均使用SPSS 22.0软件包（SPSS，美国伊利诺伊州芝加哥）进行。
ESCC患者的平均年龄±标准差为60.4±7.9岁。ESCC患者的性别、年龄、吸烟状况和上消化道癌家族史与生存时间无显著关系。rs1136410 T/T、T/C和C/C基因型携带者的平均生存时间分别为43.3、42.3和46.6个月。rs1136410与ESCC患者的生存时间无关。对于rs8679，T/T基因型携带者的平均生存时间为43.7个月，与T/C基因型患者（42.1个月）无显著差异。
在中国北方磁县高发地区，rs1136410和rs8679 SNP可能无法用于预测ESCC患者的生存情况。有必要探索PARP1基因的其他SNP是否对ESCC患者的预后有影响。
