PARP-1 Val762Ala 多态性与韩国男性非霍奇金淋巴瘤风险降低相关。
PARP-1 Val762Ala polymorphism is associated with reduced risk of non-Hodgkin lymphoma in Korean males.
机构信息
Genome Research Center for Hematopoietic Diseases, Chonnam National University Hwasun Hospital, 160, Ilsim-ni, Hwasun-eup, Hwasun-gun, Chonnam, 519-809, Republic of Korea.
出版信息
BMC Med Genet. 2010 Mar 3;11:38. doi: 10.1186/1471-2350-11-38.
BACKGROUND
Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays a role in DNA repair, differentiation, proliferation, and cell death. The polymorphisms of PARP-1 have been associated with the risk of various carcinomas, including breast, lung, and prostate. We investigated whether PARP-1 polymorphisms are associated with the risk of non-Hodgkin lymphoma (NHL).
METHODS
Subjects from a Korean population consisting of 573 NHL patients and 721 controls were genotyped for 5 PARP-1 polymorphisms (Asp81Asp, Ala284Ala, Lys352Lys, IVS13+118A>G, and Val762Ala) using High Resolution Melting polymerase chain reaction (PCR) and an automatic sequencer.
RESULTS
None of the 5 polymorphisms were associated with overall risk for NHL. However, the Val762Ala polymorphism was associated with reduced risk for NHL in males [odds ratio (OR), 0.62; 95% confidence interval (CI), 0.41-0.93 for CC genotype and OR, 0.84; 95% CI, 0.60-1.16 for TC genotype] with a trend toward a gene dose effect (p for trend, 0.02). The Asp81Asp (p for trend, 0.04) and Lys352Lys (p for trend, 0.03) polymorphisms revealed the same trend. In an association study of PARP-1 haplotypes, the haplotype-ACAAC was associated with decreased risk of NHL in males (OR, 0.75; 95% CI, 0.59-0.94).
CONCLUSION
The present data suggest that Val762Ala, Asp81Asp, and Lys352Lys polymorphisms and the haplotype-ACAAC in PARP-1 are associated with reduced risk of NHL in Korean males.
背景
多聚（ADP-核糖）聚合酶-1（PARP-1）是一种核酶，在 DNA 修复、分化、增殖和细胞死亡中发挥作用。PARP-1 的多态性与各种癌的风险相关，包括乳腺癌、肺癌和前列腺癌。我们研究了 PARP-1 多态性是否与非霍奇金淋巴瘤（NHL）的风险相关。
方法
来自韩国人群的 573 例 NHL 患者和 721 例对照者的研究对象使用高分辨率熔解聚合酶链反应（PCR）和自动测序仪对 5 个 PARP-1 多态性（Asp81Asp、Ala284Ala、Lys352Lys、IVS13+118A>G 和 Val762Ala）进行了基因分型。
结果
这 5 个多态性均与 NHL 的总体风险无关。然而，Val762Ala 多态性与男性 NHL 风险降低相关[优势比（OR），0.62；95%置信区间（CI），0.41-0.93 对于 CC 基因型和 OR，0.84；95%CI，0.60-1.16 对于 TC 基因型]，且存在基因剂量效应的趋势（趋势检验的 P 值为 0.02）。Asp81Asp（P 趋势，0.04）和 Lys352Lys（P 趋势，0.03）多态性也呈现出相同的趋势。在 PARP-1 单倍型的关联研究中，ACAAC 单倍型与男性 NHL 风险降低相关（OR，0.75；95%CI，0.59-0.94）。
结论
本研究数据表明，PARP-1 中的 Val762Ala、Asp81Asp 和 Lys352Lys 多态性以及 ACAAC 单倍型与韩国男性 NHL 风险降低相关。
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