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β-环糊精包合对淫羊藿苷溶解度和酶解速率的影响。

Effect of β-cyclodextrin complexation on solubility and enzymatic hydrolysis rate of icariin.

作者信息

Cui Li, Zhang Zhenhai, Sun E, Jia Xiaobin, Qian Qian

机构信息

Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, China.

出版信息

J Nat Sci Biol Med. 2013 Jan;4(1):201-6. doi: 10.4103/0976-9668.107291.

DOI:10.4103/0976-9668.107291
PMID:23633863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3633278/
Abstract

OBJECTIVE

The aim of this work was to investigate the effect of β-cyclodextrin complexation on the solubility and hydrolysis rate of icariin.

MATERIAL AND METHODS

The inclusion complex of icariin at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of icariin complex in water at 37°C was 36 times greater than that of free icariin. Enzymatic hydrolysis conditions were tested for the bioconversion of icariin by mono-factor experimental design.

METHODS

The inclusion complex of icariin at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of icariin complex in water at 37°C was 36 times greater than that of free icariin. Enzymatic hydrolysis conditions were tested for the bioconversion of icariin by mono-factor experimental design.

RESULTS

The enzymatic hydrolysis experiment showed that icariin can be transformed into baohuoside I. The optimum conditions determined were as follows: pH 5.0, 50°C, the ratio of cellulase/substrate (0.6), the concentration of icariin 20 mg/ml, and reaction time 12 h. Under these enzymatic conditions, 98.2% transforming rate of baohuoside I from icariin in inclusion complexes was obtained.

CONCLUSION

The aqueous solubility and enzymatic hydrolysis rate of icariin were improved owing to the inclusion complexation.

摘要

目的

本研究旨在探讨β-环糊精包合作用对淫羊藿苷溶解度和水解速率的影响。

材料与方法

采用滴加法制备了摩尔比为1:1的淫羊藿苷包合物，并用差示扫描量热法对其进行了表征。淫羊藿苷包合物在37℃水中的溶解度比游离淫羊藿苷高36倍。通过单因素实验设计对淫羊藿苷生物转化的酶解条件进行了测试。

方法

采用滴加法制备了摩尔比为1:1的淫羊藿苷包合物，并用差示扫描量热法对其进行了表征。淫羊藿苷包合物在37℃水中的溶解度比游离淫羊藿苷高36倍。通过单因素实验设计对淫羊藿苷生物转化的酶解条件进行了测试。

结果

酶解实验表明，淫羊藿苷可转化为宝藿苷I。确定的最佳条件如下：pH 5.0、50℃、纤维素酶/底物比（0.6）、淫羊藿苷浓度20 mg/ml、反应时间12 h。在这些酶解条件下，包合物中淫羊藿苷转化为宝藿苷I的转化率为98.2%。

结论

包合作用提高了淫羊藿苷的水溶性和酶解速率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/d6eef526118b/JNSBM-4-201-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/2d1c3d8fc4dc/JNSBM-4-201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/76311713968b/JNSBM-4-201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/45d5f075064f/JNSBM-4-201-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/079ebd527667/JNSBM-4-201-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/01e777db09a2/JNSBM-4-201-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/086057fa1fe7/JNSBM-4-201-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/d21d8c1a4862/JNSBM-4-201-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/d6eef526118b/JNSBM-4-201-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/2d1c3d8fc4dc/JNSBM-4-201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/76311713968b/JNSBM-4-201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/45d5f075064f/JNSBM-4-201-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/079ebd527667/JNSBM-4-201-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/01e777db09a2/JNSBM-4-201-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/086057fa1fe7/JNSBM-4-201-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/d21d8c1a4862/JNSBM-4-201-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fce/3633278/d6eef526118b/JNSBM-4-201-g009.jpg

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