Huang Yaw-Bin, Lin Ming-Wei, Chao Yun, Huang Chi-Te, Tsai Yi-Hung, Wu Pao-Chu
Graduate Institute of Clinical Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
Int J Urol. 2014 Jan;21(1):94-8. doi: 10.1111/iju.12179. Epub 2013 May 2.
To evaluate the anti-oxidant activity of the flavonoid compound, kaempferol, and to examine its role in the suppression of oxidative stress and attenuation of bladder hyperactivity in a rat model of bladder injury.
The anti-oxidative activity of kaempferol was examined in lipopolysaccharide-treated RAW264.7 macrophages by using flow cytometry. For in vivo studies, rats were pretreated with kaempferol or vehicle for 24 h. The rat urothelium was injured by the administration of protamine sulfate for 1.5 h and irritated by the subsequent infusion of potassium chloride for 4 h. Oxidative stress in the bladder tissue was assessed using chemiluminescence assay, and the bladder pressure was determination by cystomertrogram.
Kaempferol significantly suppressed lipopolysaccharide-induced reactive oxygen species production in RAW264.7 rat macrophages. Exposure of the rat bladder to sequential infusion of protamine sulfate and potassium chloride induced bladder hyperactivity. Pretreatment with kaempferol, prevented the formation of reactive oxygen species and prolonged the intercontraction interval.
Kaempferol suppresses oxidative stress and attenuates bladder hyperactivity caused by potassium chloride after protamine sulfate-induced bladder injury.
评估黄酮类化合物山奈酚的抗氧化活性，并研究其在膀胱损伤大鼠模型中抑制氧化应激和减轻膀胱活动亢进的作用。
采用流式细胞术检测山奈酚在脂多糖处理的RAW264.7巨噬细胞中的抗氧化活性。在体内研究中，大鼠用山奈酚或赋形剂预处理24小时。通过给予硫酸鱼精蛋白1.5小时损伤大鼠尿路上皮，并随后输注氯化钾4小时进行刺激。使用化学发光法评估膀胱组织中的氧化应激，并通过膀胱压力测定仪测定膀胱压力。
山奈酚显著抑制脂多糖诱导的RAW264.7大鼠巨噬细胞中活性氧的产生。大鼠膀胱先后输注硫酸鱼精蛋白和氯化钾会诱导膀胱活动亢进。用山奈酚预处理可防止活性氧的形成并延长收缩间期。
山奈酚可抑制氧化应激，并减轻硫酸鱼精蛋白诱导的膀胱损伤后由氯化钾引起的膀胱活动亢进。
