Ciucchi Philip, Neuhaus Klaus W, Emerich Marta, Peutzfeldt Anne, Lussi Adrian
Department of Operative, Preventive and Paediatric Dentistry, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland,
Lasers Med Sci. 2015 Jan;30(1):1-9. doi: 10.1007/s10103-013-1333-2. Epub 2013 May 1.
The aim of the study was to compare fissure sealant quality after mechanical conditioning of erbium-doped yttrium aluminium garnet (Er:YAG) laser or air abrasion prior to chemical conditioning of phosphoric acid etching or of a self-etch adhesive. Twenty-five permanent molars were initially divided into three groups: control group (n = 5), phosphoric acid etching; test group 1 (n = 10), air abrasion; and test group 2, (n = 10) Er:YAG laser. After mechanical conditioning, the test group teeth were sectioned buccolingually and the occlusal surface of one half tooth (equal to one sample) was acid etched, while a self-etch adhesive was applied on the other half. The fissure system of each sample was sealed, thermo-cycled and immersed in 5% methylene dye for 24 h. Each sample was sectioned buccolingually, and one slice was analysed microscopically. Using specialized software microleakage, unfilled margin, sealant failure and unfilled area proportions were calculated. A nonparametric ANOVA model was applied to compare the Er:YAG treatment with that of air abrasion and the self-etch adhesive with phosphoric acid (α = 0.05). Test groups were compared to the control group using Wilcoxon rank sum tests (α = 0.05). The control group displayed significantly lower microleakage but higher unfilled area proportions than the Er:YAG laser + self-etch adhesive group and displayed significantly higher unfilled margin and unfilled area proportions than the air-abrasion + self-etch adhesive group. There was no statistically significant difference in the quality of sealants applied in fissures treated with either Er:YAG laser or air abrasion prior to phosphoric acid etching, nor in the quality of sealants applied in fissures treated with either self-etch adhesive or phosphoric acid following Er:YAG or air-abrasion treatment.
本研究的目的是比较在进行磷酸酸蚀或自酸蚀粘结剂化学处理之前，掺铒钇铝石榴石（Er:YAG）激光或空气喷砂机械预处理后窝沟封闭剂的质量。25颗恒牙最初被分为三组：对照组（n = 5），采用磷酸酸蚀；试验组1（n = 10），采用空气喷砂；试验组2（n = 10），采用Er:YAG激光。机械预处理后，将试验组牙齿沿颊舌向切开，一半牙齿（相当于一个样本）的咬合面进行酸蚀，另一半则应用自酸蚀粘结剂。每个样本的窝沟系统进行封闭、热循环处理，并在5%亚甲基蓝染料中浸泡24小时。每个样本沿颊舌向切开，取一片进行显微镜分析。使用专业软件计算微渗漏、未填充边缘、封闭剂失败和未填充面积比例。应用非参数方差分析模型比较Er:YAG处理与空气喷砂处理以及自酸蚀粘结剂与磷酸酸蚀的效果（α = 0.05）。使用Wilcoxon秩和检验将试验组与对照组进行比较（α = 0.05）。对照组的微渗漏显著低于Er:YAG激光+自酸蚀粘结剂组，但未填充面积比例高于该组；对照组的未填充边缘和未填充面积比例显著高于空气喷砂+自酸蚀粘结剂组。在磷酸酸蚀之前用Er:YAG激光或空气喷砂处理的窝沟中应用的封闭剂质量，以及在Er:YAG或空气喷砂处理之后用自酸蚀粘结剂或磷酸酸蚀处理的窝沟中应用的封闭剂质量，均无统计学显著差异。
