School of Veterinary Sciences, University of Bristol, Langford, Bristol, BS40 5DU, UK.
Curr Microbiol. 2013 Sep;67(3):333-40. doi: 10.1007/s00284-013-0370-1. Epub 2013 May 1.
Control of Campylobacter in the food chain requires a better understanding of the behaviour of the bacteria in relevant environments. Campylobacter species are largely non-pathogenic in poultry, the body temperature of which is 42 °C. However, the bacteria are highly pathogenic in humans whose body temperature is 37 °C. The aim of this study was to examine if switching from commensal to pathogenic behaviour was related to temperature. We examined the growth, motility and invasion of T84 cells by three species of Campylobacter: C. jejuni 81116, C. jejuni M1, C. coli 1669, C. coli RM2228 and C. fetus fetus NC10842 grown at 37 and 42 °C. Our results suggest that C. jejuni isolates grow similarly at both temperatures but some are more motile at 42 °C and some are more invasive at 37 °C, which may account for its rapid spread in poultry flocks and for infection in humans, respectively. C. coli, which are infrequent causes of Campylobacter infections in humans, is less able to grow and move at 37 °C compared to 42 °C but was significantly more invasive at the lower temperature. C. fetus fetus, which is infrequently found in poultry, is less able to grow and invade at 42 °C.
控制食物链中的弯曲杆菌需要更好地了解细菌在相关环境中的行为。在禽类中，弯曲杆菌属的大多数物种基本上是无害的，因为禽类的体温为 42°C。然而，在人体中，这些细菌具有高度致病性，因为人体的体温为 37°C。本研究旨在探讨从共生行为向致病性行为的转变是否与温度有关。我们检查了三种弯曲杆菌（空肠弯曲杆菌 81116、空肠弯曲杆菌 M1、大肠弯曲杆菌 1669、大肠弯曲杆菌 RM2228 和胎儿弯曲杆菌 NC10842）在 37°C 和 42°C 下的生长、运动和对 T84 细胞的侵袭能力。我们的结果表明，两种温度下，空肠弯曲杆菌分离株的生长情况相似，但有些在 42°C 时运动性更强，有些在 37°C 时侵袭性更强，这可能分别解释了其在禽类中的快速传播和在人类中的感染。大肠弯曲杆菌在人类中很少引起弯曲杆菌感染，与 42°C 相比，其在 37°C 下的生长和运动能力较差，但在较低温度下侵袭性更强。胎儿弯曲杆菌在禽类中很少发现，其在 42°C 下的生长和侵袭能力较差。
