a Animal Physiology Laboratory, Research Unit of Toxicology and Environmental Health, Sfax Faculty of Sciences , University of Sfax , Sfax , Tunisia.
b Biochemistry Laboratory , CHU Hedi Chaker, University of Sfax , Sfax , Tunisia.
Arch Physiol Biochem. 2018 May;124(2):175-184. doi: 10.1080/13813455.2017.1371195. Epub 2017 Sep 6.
Data on the individual nephrotoxic effects of imidacloprid (IMI) and gibberellic acid (GA) are scarce. Moreover, there is a lack of information about their combined effects on the renal tissue. Our study investigated the effects of IMI and GA separately or together on rats kidney. IMI (64 mg/kg bw) was given for 3 weeks by gavage either individually or in combination with GA (200 mg/L) via drinking water. IMI associated or no with GA increased the levels of kidney malondialdehyde, advanced oxidation protein products, protein carbonyls and metallothionein, plasma creatinine, urea, blood urea nitrogen and lactate dehydrogenase activity. A decline of kidney uric acid level and antioxidant status was also observed. All these changes were supported by histopathological observations. Our results highlighted the role of IMI and/or GA-induced nephrotoxicity. Co-exposure to IMI and GA exhibited synergism in biochemical kidney variables and histopathology and antagonism in physical and morphological parameters.
关于吡虫啉（IMI）和赤霉素（GA）对肾脏的单独毒性作用的数据很少。此外，关于它们对肾脏组织的联合作用的信息也很缺乏。我们的研究分别调查了 IMI 和 GA 单独或联合对大鼠肾脏的影响。通过灌胃给予 IMI（64mg/kg bw），连续 3 周；或者通过饮用水给予 IMI（64mg/kg bw）联合 GA（200mg/L）。结果发现，IMI 联合或不联合 GA 增加了肾脏丙二醛、晚期氧化蛋白产物、蛋白质羰基和金属硫蛋白、血浆肌酐、尿素、血尿素氮和乳酸脱氢酶活性的水平。还观察到肾脏尿酸水平和抗氧化状态的下降。这些变化都得到了组织病理学观察的支持。我们的结果强调了 IMI 和/或 GA 诱导的肾毒性的作用。IMI 和 GA 的联合暴露在生化肾变量和组织病理学方面表现出协同作用，而在物理和形态参数方面则表现出拮抗作用。
