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纳米壳聚糖基 DNA 疫苗编码 Esat-6 和 FL 的 T 细胞表位对结核分枝杆菌感染的增强免疫应答和保护作用。

Enhanced immune response and protective effects of nano-chitosan-based DNA vaccine encoding T cell epitopes of Esat-6 and FL against Mycobacterium tuberculosis infection.

机构信息

Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, China.

出版信息

PLoS One. 2013 Apr 23;8(4):e61135. doi: 10.1371/journal.pone.0061135. Print 2013.

DOI:10.1371/journal.pone.0061135
PMID:23637790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3634041/
Abstract

Development of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nano-chitosan). The immunologic and protective efficacy of the nano-chitosan-based DNA vaccine (termed nano-Esat-6/3e-FL) was assessed in C57BL/6 mice after intramuscular prime vaccination with the plasmids DNA and nasal boost with the Esat-6/3e peptides. The results showed that the immunized mice remarkably elicited enhanced T cell responses and protection against M.tb H37Rv challenge. These findings indicate that the nano-chitosan can significantly elevate the immunologic and protective effects of the DNA vaccine, and the nano-Esat-6/3e-FL is a useful vaccine for preventing M.tb infection in mice.

摘要

开发针对结核分枝杆菌（M.tb）的新型有效疫苗是预防 TB 感染的一项挑战。本研究开发了一种新型的基于纳米粒子的重组 DNA 疫苗，该疫苗包含 Esat-6 三个 T 细胞表位（Esat-6/3e）和 fms 样酪氨酸激酶 3 配体（FL）基因（称为 Esat-6/3e-FL），并被壳聚糖（CS）纳米粒子（纳米壳聚糖）包裹。通过肌肉内初次免疫接种质粒 DNA 和鼻内加强 Esat-6/3e 肽，评估了基于纳米壳聚糖的 DNA 疫苗（称为纳米-Esat-6/3e-FL）在 C57BL/6 小鼠中的免疫和保护效力。结果表明，免疫小鼠显著增强了针对 M.tb H37Rv 挑战的 T 细胞反应和保护作用。这些发现表明，纳米壳聚糖可以显著提高 DNA 疫苗的免疫和保护效果，纳米-Esat-6/3e-FL 是预防小鼠 M.tb 感染的有效疫苗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/bcfbdaabec3f/pone.0061135.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/bd1b0b6d39ed/pone.0061135.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/9ec5d68ba728/pone.0061135.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/ec8f59dbba52/pone.0061135.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/92a029afd1e3/pone.0061135.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/bcfbdaabec3f/pone.0061135.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/bd1b0b6d39ed/pone.0061135.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/5825810fd549/pone.0061135.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/9ec5d68ba728/pone.0061135.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/ec8f59dbba52/pone.0061135.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/92a029afd1e3/pone.0061135.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d2/3634041/bcfbdaabec3f/pone.0061135.g006.jpg

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