用高压液相色谱法纯化葡萄球菌剥脱毒素

Purification of staphylococcal exfoliative toxin by high pressure liquid chromatography.

作者信息

Sugai M, Inoue S, Hino T, Kuwabara M, Hong Y M, Miyake Y, Suginaka H

机构信息

Department of Microbiology, Hiroshima University, School of Dentistry, Japan.

出版信息

Zentralbl Bakteriol. 1990 May;273(1):5-11. doi: 10.1016/s0934-8840(11)80234-3.

DOI:10.1016/s0934-8840(11)80234-3

PMID:2363797

Abstract

Exfoliative toxin (ET) isolated from a clinical strain of Staphylococcus aureus was purified to homogeneity, using a 3-step HPLC system. NH2-terminal 20 amino residues of purified ET was found to be identical with ETA of S. aureus TA (7), S. aureus TC16 (9) and S. aureus ZM (10), but stability of purified ET was completely different from that of ETA. This purification system gave a high yield of pure ET, which exhibited higher purity than specimens purified by more complicated and time-consuming procedures. It is useful for small-scale purification for the comparative study of ET and easy to scale up for preparative purification.

摘要

使用三步高效液相色谱系统，从金黄色葡萄球菌临床菌株中分离出的剥脱毒素（ET）被纯化至同质。纯化后的ET的氨基末端20个氨基酸残基与金黄色葡萄球菌TA（7）、金黄色葡萄球菌TC16（9）和金黄色葡萄球菌ZM（10）的ETA相同，但纯化后的ET的稳定性与ETA完全不同。该纯化系统可获得高产率的纯ET，其纯度高于通过更复杂且耗时的程序纯化的标本。它对于ET的比较研究的小规模纯化很有用，并且易于扩大规模进行制备性纯化。