西红花水提物抑制 Wistar 白化大鼠化学诱导的胃癌进展。

OBJECTIVE(S): Gastric cancer is the first and second leading cause of cancer related death in Iranian men and women, respectively. Gastric cancer management is based on the surgery, radiotherapy and chemotherapy. In the present study, for the first time, the beneficial effect of saffron (Crocus sativus L.) aqueous extract (SAE) on the 1-Methyl -3- nitro -1- nitrosoguanidine (MNNG)-induced gastric cancer in rat was investigated.

MATERIALS AND METHODS

MNNG was used to induce gastric cancer and then, different concentrations of SAE were administered to rats. After sacrificing, the stomach tissue was investigated by both pathologist and flow cytometry, and several biochemical parameters was determined in the plasma (or serum) and stomach of rats.

RESULTS

Pathologic data indicated the induction of cancer at different stages from hyperplasia to adenoma in rats; and the inhibition of cancer progression in the gastric tissue by SAE administration; so that, 20% of cancerous rats treated with higher doses of SAE was completely normal at the end of experiment and there was no rat with adenoma in the SAE treated groups. In addition, the results of the flow cytometry/ propidium iodide staining showed that the apoptosis/proliferation ratio was increased due to the SAE treatment of cancerous rats. Moreover, the significantly increased serum LDH and decreased plasma antioxidant activity due to cancer induction fell backwards after treatment of rats with SAE. But changes in the other parameters (Ca(2+), tyrosine kinase activity and carcino-embryonic antigen) were not significant.

CONCLUSION

SAE inhibits the progression of gastric cancer in rats, in a dose dependent manner.

目的

胃癌分别是伊朗男性和女性癌症相关死亡的第一和第二大原因。胃癌的治疗基于手术、放疗和化疗。在本研究中，首次研究了藏红花（Crocus sativus L.）水提取物（SAE）对大鼠 1-甲基-3-硝基-1-亚硝基胍（MNNG）诱导的胃癌的有益作用。

材料和方法

MNNG 用于诱导胃癌，然后给大鼠给予不同浓度的 SAE。处死大鼠后，病理学家和流式细胞术同时对胃组织进行检查，并测定大鼠血浆（或血清）和胃中的几种生化参数。

结果

病理数据表明，在大鼠中诱导了从增生到腺瘤的不同阶段的癌症；并且 SAE 给药抑制了胃癌的进展；因此，用较高剂量 SAE 治疗的 20%的癌变大鼠在实验结束时完全正常，SAE 治疗组中没有大鼠患有腺瘤。此外，流式细胞术/碘化丙啶染色的结果表明，由于对癌变大鼠进行了 SAE 治疗，凋亡/增殖比增加。此外，由于癌症诱导，血清 LDH 显著增加，血浆抗氧化活性降低，但大鼠用 SAE 治疗后，这些参数又恢复正常。但是其他参数（Ca(2+)、酪氨酸激酶活性和癌胚抗原）的变化不显著。