Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Ultrasound Med Biol. 2011 May;37(5):780-7. doi: 10.1016/j.ultrasmedbio.2010.11.015. Epub 2011 Mar 31.
Gastric cancer is often diagnosed at advanced stages and there is no accurate method for its screening and diagnosis, especially in small animals. Here, we explain the application of B-mode ultrasound imaging (BMUI) for screening of gastric changes in the rat. Thus, male Albino Wistar rats, weighing 100-120 grams were randomly divided into two groups. The control group rats (n=10) were given water as routine; the remaining (n=90), were given N-methyl-N-nitro-N-nitrosoguanidine (MNNG, 100 μg/mL) in drinking water ad libitum for 40 weeks. Fifteen rats were killed at different time intervals and the others were sacrificed after 55 weeks. The BMUI of the stomach of animals after MNNG administration show some changes compared with the normal groups. Pathologic investigations of the stomach indicate cancer induction at different levels. The sensitivity and specificity of BMUI is 96.6% and 78.78%, respectively. Thus, it is a useful method of diagnosis of gastric cancer in rats.
胃癌通常在晚期诊断，目前尚无准确的筛查和诊断方法，尤其是在小动物中。在这里，我们解释了 B 型超声成像 (BMUI) 在大鼠胃变化筛查中的应用。因此，将体重为 100-120 克的雄性白化 Wistar 大鼠随机分为两组。对照组大鼠 (n=10) 给予常规水；其余 (n=90) 给予 N-甲基-N-亚硝基-N-亚硝胍 (MNNG，100μg/mL) 在饮水中自由饮用 40 周。15 只大鼠在不同时间间隔处死，其余大鼠在 55 周后处死。MNNG 给药后动物胃的 BMUI 与正常组相比显示出一些变化。胃的病理检查表明不同程度的癌症诱导。BMUI 的灵敏度和特异性分别为 96.6%和 78.78%。因此，它是诊断大鼠胃癌的一种有用方法。
