Department of Physiology, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, Jiangsu 221002, China.
Int Immunopharmacol. 2013 Jun;16(2):332-40. doi: 10.1016/j.intimp.2013.04.017. Epub 2013 Apr 30.
Lupeol, a triterpene, was reported to possess beneficial effects as a therapeutic and preventive agent for a range of disorders. Many studies have confirmed that lupeol possesses strong activities such as antioxidative, antiinflammatory, antiarthritic, antimutagenic, and antimalarial, both in vitro and in vivo, and at its effective therapeutic doses exhibit no toxicity to normal cells and tissues. Lupeol was observed to inhibit the proliferation of gastric tumour cells in a dose-dependent manner, as assessed by MTT assay, and induce the proliferation of NK cells, as assessed by flow cytometry and Western blotting. The killing effect of NK cells on gastric tumour cells was assessed by LDH. Our experiment demonstrated that lupeol at appropriate concentrations could promote the proliferation of NK cells, inhibit the proliferation of gastric cancer cell lines BGC823, N87 and HGC27, and increase the killing effect of NK cells on gastric cancer cells. We speculated that lupeol might increase the expression of PFP, IFN-γ, and CD107a via the activation of PI3K/Akt and Wnt/β-catenin signalling pathways. Lupeol could serve as a potential agent against gastric cancer; however, further in-depth in vivo studies are still required.
羽扇豆醇是一种三萜类化合物，据报道具有治疗和预防多种疾病的有益作用。许多研究已经证实，羽扇豆醇具有强大的活性，如抗氧化、抗炎、抗关节炎、抗突变和抗疟疾，无论是在体外还是体内，在有效的治疗剂量下，对正常细胞和组织没有毒性。MTT 检测结果表明，羽扇豆醇能够以剂量依赖的方式抑制胃肿瘤细胞的增殖，流式细胞术和 Western blot 检测结果表明，羽扇豆醇能够诱导 NK 细胞的增殖。LDH 检测结果表明，NK 细胞对胃肿瘤细胞的杀伤作用。我们的实验表明，适当浓度的羽扇豆醇能够促进 NK 细胞的增殖，抑制胃癌细胞系 BGC823、N87 和 HGC27 的增殖，并增加 NK 细胞对胃癌细胞的杀伤作用。我们推测，羽扇豆醇可能通过激活 PI3K/Akt 和 Wnt/β-catenin 信号通路，增加 PFP、IFN-γ 和 CD107a 的表达。羽扇豆醇可能是一种有潜力的胃癌治疗药物；然而，仍需要进一步的深入体内研究。
