Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
Methods. 2013 Aug 1;62(2):171-6. doi: 10.1016/j.ymeth.2013.04.018. Epub 2013 Apr 29.
S-nitrosylation of protein cysteine residues is known to be an important mechanism for nitric oxide signaling. However, the detection of protein S-nitrosylation is still challenging due to technical limitations of current methods. This chapter provides a brief review on recent developments of methods, which directly target S-nitroso moieties for detection. We also describe in detail the protocol of an organophosphine-based biotin labeling of protein S-nitroso moieties.
蛋白质半胱氨酸残基的 S-亚硝基化被认为是一氧化氮信号转导的重要机制。然而，由于当前方法的技术限制，蛋白质 S-亚硝基化的检测仍然具有挑战性。本章简要综述了近年来直接针对 S-亚硝基部分进行检测的方法的最新进展。我们还详细描述了基于有机膦的蛋白质 S-亚硝基化部分生物素标记的方案。
