Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
Curr Opin Chem Biol. 2011 Feb;15(1):32-7. doi: 10.1016/j.cbpa.2010.10.006. Epub 2010 Oct 29.
Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.
一氧化氮（NO）是一种参与多种生理和病理生理过程的细胞信号分子。NO（或其代谢物）对半胱氨酸残基的修饰，即 S-亚硝化，改变了广泛的蛋白质的功能。该反应代表一种重要的翻译后修饰，可传递 NO 依赖性信号。然而，生物样品中 S-亚硝化的检测和定量仍然是一个挑战，主要是因为 S-亚硝化产物（SNO）的不稳定性：S-亚硝硫醇（SNO）。在这篇综述中，我们总结了检测 S-亚硝化的方法的最新进展。我们的重点是可用于直接连接 S-亚硝化部位的方法。
