通过还原连接介导的 S-亚硝基硫醇一步形成二硫键。
Reductive ligation mediated one-step disulfide formation of S-nitrosothiols.
机构信息
Department of Chemistry, Washington State University, Pullman, Washington 99164, USA.
出版信息
Org Lett. 2010 Sep 17;12(18):4208-11. doi: 10.1021/ol101863s.
Abstract
A one-step reductive ligation mediated disulfide formation of S-nitrosothiols was developed. This reaction involves the reaction of the S-nitroso group with phosphine-thioesters to form sulfenamide and thiolate intermediates, which then undergo a fast intermolecular disulfide formation to form stable conjugates. This reaction can be used to design new biosensors of S-nitrosated proteins.
摘要
开发了一种一步还原连接介导的 S-亚硝基硫醇的二硫键形成方法。该反应涉及 S-亚硝基基团与膦硫酯的反应，形成磺酰胺和硫醇中间体，然后快速进行分子间二硫键形成，形成稳定的缀合物。该反应可用于设计新的 S-硝化蛋白生物传感器。
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