Sauermann U, Schneider J, Mous J, Brunckhorst U, Schedel I, Jentsch K D, Hunsmann G
German Primate Center, Department of Virology and Immunology, Göttingen, Federal Republic of Germany.
AIDS Res Hum Retroviruses. 1990 Jun;6(6):813-23. doi: 10.1089/aid.1990.6.813.
The genetic diversity of HIV-1 is well documented. Except for the HIV-1 isolate LAV-1BRU, no nucleic acid sequence of a European isolate of HIV-1 has been published to date. To further investigate the extent of the genetic variability and the evolution of HIV-1, we have isolated, cloned, and subsequently sequenced HIV-1 from a German patient with AIDS-related complex. Comparative studies of the nucleic acid sequence revealed that this isolate, designated HAN2, is highly divergent from the North American and African subtypes of HIV-1 and may represent a European subtype of HIV-1. Furthermore, a full-length molecular clone was derived from this isolate which was infectious in human T-cell lines. Therefore this new isolate will be particularly useful for studies on the genetic evolution and biology of HIV-1 as well as for testing antiviral substances and for developing vaccines.
HIV-1的基因多样性已有充分记载。除了HIV-1分离株LAV-1BRU外，迄今为止，尚未发表过欧洲HIV-1分离株的核酸序列。为了进一步研究HIV-1的基因变异性程度及其进化，我们从一名患有艾滋病相关综合征的德国患者身上分离、克隆并随后对HIV-1进行了测序。核酸序列的比较研究表明，这种命名为HAN2的分离株与HIV-1的北美和非洲亚型高度不同，可能代表HIV-1的一种欧洲亚型。此外，从该分离株获得了一个全长分子克隆，它在人T细胞系中具有感染性。因此，这种新的分离株对于HIV-1的基因进化和生物学研究、抗病毒物质测试以及疫苗开发将特别有用。
