Department of Biomedical Engineering, College of Engineering, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China.
Sci Rep. 2013;3:1762. doi: 10.1038/srep01762.
Ischemia-reperfusion (I/R) injury model has been widely applied to the study of microcirculation disturbance. In this work, we used laser oblique scanning optical microscopy (LOSOM) to observe the microcirculation system in the mesentery of rat model. Utilizing a localized point-scanning detection scheme, high-contrast images of leukocytes were obtained. The extended detection capability facilitated both the automatic in vivo cell counting and the accurate measurement of the rolling velocity of leukocytes. Statistical analysis of the different treatment groups suggested that the distinction between I/R and sham groups with time lapse is significant.
缺血再灌注 (I/R) 损伤模型已广泛应用于微循环障碍的研究。在这项工作中，我们使用激光斜扫描光学显微镜 (LOSOM) 观察大鼠模型肠系膜中的微循环系统。利用局部点扫描检测方案，获得了高对比度的白细胞图像。扩展的检测能力不仅实现了白细胞的自动体内计数，还实现了白细胞滚动速度的精确测量。对不同治疗组的统计分析表明，I/R 组与假手术组随时间推移的差异具有统计学意义。
