Department of Medicine, David Geffen School of Medicine at UCLA, and.
Department of Bioengineering, UCLA, Los Angeles, California, USA.
JCI Insight. 2018 Aug 23;3(16). doi: 10.1172/jci.insight.121396.
The ability to image tissue morphogenesis in real-time and in 3-dimensions (3-D) remains an optical challenge. The advent of light-sheet fluorescence microscopy (LSFM) has advanced developmental biology and tissue regeneration research. In this review, we introduce a LSFM system in which the illumination lens reshapes a thin light-sheet to rapidly scan across a sample of interest while the detection lens orthogonally collects the imaging data. This multiscale strategy provides deep-tissue penetration, high-spatiotemporal resolution, and minimal photobleaching and phototoxicity, allowing in vivo visualization of a variety of tissues and processes, ranging from developing hearts in live zebrafish embryos to ex vivo interrogation of the microarchitecture of optically cleared neonatal hearts. Here, we highlight multiple applications of LSFM and discuss several studies that have allowed better characterization of developmental and pathological processes in multiple models and tissues. These findings demonstrate the capacity of multiscale light-sheet imaging to uncover cardiovascular developmental and regenerative phenomena.
实时和三维（3-D）成像组织形态发生的能力仍然是一个光学挑战。光片荧光显微镜（LSFM）的出现推动了发育生物学和组织再生研究的发展。在这篇综述中，我们介绍了一种 LSFM 系统，其中照明透镜重塑了一个薄光片，以便快速扫描感兴趣的样本，而检测透镜则正交地收集成像数据。这种多尺度策略提供了深组织穿透、高时空分辨率以及最小的光漂白和光毒性，允许对各种组织和过程进行活体可视化，从活体斑马鱼胚胎中的发育心脏到体外研究光透明新生儿心脏的微观结构。在这里，我们强调了 LSFM 的多种应用，并讨论了一些研究，这些研究使我们能够更好地描述多种模型和组织中的发育和病理过程。这些发现表明，多尺度光片成像有能力揭示心血管发育和再生现象。
