CIBIO-UP, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, InBIO, Laboratório Associado, Campus Agrário de Vairão, Rua Padre Armando Quintas, Vairão, Portugal,
Arch Virol. 2013 Oct;158(10):2193-6. doi: 10.1007/s00705-013-1714-7. Epub 2013 May 3.
European brown hare syndrome (EBHS) is characterised by high mortality of European brown hares (Lepus europaeus) and mountain hares (Lepus timidus). European brown hare syndrome virus (EBHSV) and the closely related rabbit haemorrhagic disease virus (RHDV) comprise the genus Lagovirus, family Caliciviridae. In contrast to RHDV, which is well studied, with more than 30 complete genome sequences available, the only complete genome sequence available for EBHSV was obtained from a strain isolated in 1989 in France. EBHS was originally diagnosed in Sweden in 1980. Here, we report the complete coding sequences of two EBHSV strains isolated from European brown hares that died with liver lesions characteristic of EBHS in Sweden in 1982. These sequences represent the oldest complete coding sequences of EBHSV isolated from the original area of virus diagnosis. The genomic organisation is similar to that of the published French sequence. Comparison with this sequence revealed several nucleotide substitutions, corresponding to 6 % divergence. At the amino acid level, the Swedish strains are 2 % different from the French strain. Most amino acid substitutions were located within the major capsid protein VP60, but when considering the amino acid sequence length of each protein, VP10 is the protein with the highest percentage of amino acid differences. The same result was obtained when Swedish strains were compared. This evolutionary pattern has not been described previously for members of the genus Lagovirus.
欧洲褐兔综合征 (EBHS) 的特征是欧洲褐兔 (Lepus europaeus) 和山兔 (Lepus timidus) 的死亡率很高。欧洲褐兔病毒 (EBHSV) 和密切相关的兔出血症病毒 (RHDV) 属于杯状病毒科杯状病毒属。与 RHDV 不同，后者研究得很好，有 30 多个完整的基因组序列可供使用，而唯一可用的 EBHSV 完整基因组序列是从 1989 年在法国分离的一株获得的。EBHS 最初于 1980 年在瑞典诊断出。在这里，我们报告了从 1982 年在瑞典死于具有 EBHS 特征的肝脏病变的欧洲褐兔中分离出的两种 EBHSV 株的完整编码序列。这些序列代表了从病毒诊断的原始区域分离出的最古老的 EBHSV 完整编码序列。基因组组织与已发表的法国序列相似。与该序列的比较显示了几个核苷酸替换，对应于 6%的差异。在氨基酸水平上，瑞典株与法国株有 2%的差异。大多数氨基酸替换位于主要衣壳蛋白 VP60 内，但考虑到每种蛋白质的氨基酸序列长度时，VP10 是氨基酸差异百分比最高的蛋白质。当比较瑞典株时，也得到了相同的结果。这种进化模式以前尚未在杯状病毒属的成员中描述过。
